Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,210 results for: val

Showing: 2941 - 2960 of 3210 results

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pBABE FLAG HA p100 (Ser707/711Ala)

Plasmid

#236469

Purpose

stable expression of p100 using virus infection

Depositor

Michele Pagano

Article

Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

Insert

p100 (NFKB2 Human)

Expression

Mammalian

Mutation

Ser707/711Ala

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-Hf-RfxCas13d-His

Plasmid

#234480

Purpose

Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

RfxCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Changed four alanine to valine in positions 134, …

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-547 aa

Plasmid

#222657

Purpose

Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-547 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated histidine 548 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-409 aa

Plasmid

#222658

Purpose

Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-409 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated proline 410 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-244 aa

Plasmid

#222659

Purpose

Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-244 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated asparagine 245 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

Plasmid

#222649

Purpose

For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

Plasmid

#224755

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C/C159A/C219A/C365A/C399A

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)

Plasmid

#224756

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131A/C159A/C219A/C365A/C399A

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-SPNS1-sg1

Plasmid

#198566

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg1 (SPNS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF8L2:1-631

Plasmid

#211239

Purpose

Mammalian expression of full-length human DCAF8L2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF8L2:M1-S631 (DCAF8L2 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

145-147: missing in Poly E region

Promoter

CMV

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-LIC_MAGEA3:104-314

Plasmid

#210941

Purpose

Insect expression of human MAGEA3 fragment E104 to E314. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MAGEA3:E104-E314 (MAGEA3 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SHKBP1:182-585

Plasmid

#210935

Purpose

Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SHKBP1:T182-L585 (SHKBP1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

Q507L natural variant (VAR_036714)

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TRAF7:382-670

Plasmid

#210930

Purpose

Insect expression of human TRAF7 fragment P382 to C670. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRAF7:P382-C670 (TRAF7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TLE3:472-772

Plasmid

#210928

Purpose

Insect expression of human TLE3 fragment G472 to Y772. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TLE3:G472-Y772 (TLE3 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_PAFAH1B1:86-410

Plasmid

#210923

Purpose

Insect expression of human PAFAH1B1 fragment G86 to R410. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PAFAH1B1:G86-R410 (PAFAH1B1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_UTP4:1-686

Plasmid

#210915

Purpose

Insect expression of full-length human UTP4. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

UTP4:M1-R686 (CIRH1A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_DCAF13:54-356

Plasmid

#210908

Purpose

Insect expression of human DCAF13 fragment F54 to A356. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF13:F54-A356 (DCAF13 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

A86S sequence conflict

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_ARPC1B:1-372

Plasmid

#210905

Purpose

Insect expression of full-length human ARPC1B. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ARPC1B:M1-K372 (ARPC1B Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_RACK1:1-317

Plasmid

#210890

Purpose

Insect expression of full-length human RACK1. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RACK1:M1-R317 (RACK1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SMU1:170-513

Plasmid

#210882

Purpose

Insect expression of human SMU1 fragment K170 to P513. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SMU1:K170-P513 (SMU1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Showing: 2941 - 2960 of 3210 results

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