We narrowed to 19,813 results for: Ada

1. G12-CASE

   Plasmid

   #190714

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.

   Inserts

   GNB3 (GNB3 Human)
   GNGT9
   GNA12 (GNA12 Human)
   

   Use

   Luciferase

   Tags

   Nluc is inserted at A133/F134 within GNA12 and cp…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-Flag-SPRTN-wt

   Plasmid

   #110214

   Purpose

   Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

   Depositor

   Kristijan Ramadan, Ivana Marinovic-Terzic

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P296L (see depositor comments below)

   Promoter

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-NAT-FLAG-CIP2A FL (sg2R)

   Plasmid

   #222624

   Purpose

   Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1-alpha promoter

   Available Since

   July 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

   Plasmid

   #223115

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

   Plasmid

   #223116

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GST-GGA3-VHS-GAT

   Plasmid

   #173719

   Purpose

   VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

   Depositor

   Juan Bonifacino

   Article

   Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

   Insert

   GGA3 (GGA3, GGA2, GGA1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT/T0-Flag-CIP2A

   Plasmid

   #222623

   Purpose

   mammalian expression vector of Flag-tagged CIP2A

   Depositor

   Daniel Durocher

   Article

   Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCMV-T7-ABE8e-SpCas9(ARGIMR)-P2A-EGFP (AHK507)

   Plasmid

   #223118

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(ARGIMR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/ARGIMR)=D1135A, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-ABE8e-SpCas9(LWQYQH)-P2A-EGFP (AHK504)

   Plasmid

   #223119

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWQYQH amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(LWQYQH)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/LWQYQH)=D1135L, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-ABE8e-SpCas9(MWKYQS)-P2A-EGFP (AHK505)

    Plasmid

    #223120

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MWKYQS)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-ABE8e-SpCas9(MWKYQA)-P2A-EGFP (AHK503)

    Plasmid

    #223122

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQA amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MWKYQA)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MWKYQA)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

    Plasmid

    #223112

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8e-SpCas9(MRKCRS)-P2A-EGFP (BKS1034)

    Plasmid

    #223117

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MRKCRS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MRKCRS)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-Lamin-A

    Plasmid

    #206027

    Purpose

    Expresses GFP-tagged Lamin A in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Lamin-A (LMNA Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Gq-CASE

    Plasmid

    #168125

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F124/E125 within GNAQ

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Gi1-CASE

    Plasmid

    #168120

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A121/E122 within GNAI1

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Gi3-CASE

    Plasmid

    #168122

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A114/E115 within GNAI1

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. G15-CASE

    Plasmid

    #168126

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at E244/N245 within GNA15

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Gi2-CASE

    Plasmid

    #168121

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at C112/E115 within GNAI2

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. G13-CASE

    Plasmid

    #168127

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F125/D126 within GNA13

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge