We narrowed to 24,706 results for: expression vector

1. pET PPL His6 LIC cloning vector (2J-T)

   Plasmid

   #37182

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and PPL

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-MCS(extended)-Puro (empty vector)

   Plasmid

   #172603

   Purpose

   Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The multiple cloning site (MCS) has been edited/e…

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ai65(RCFL-tdT) targeting vector

   Plasmid

   #61577

   Purpose

   Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   pCAG-FSF-LSL-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ai66(RCRL-tdT) targeting vector

   Plasmid

   #61578

   Purpose

   Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   CAG-RSR-LSL-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ai95(RCL-GCaMP6f) targeting vector

   Plasmid

   #61579

   Purpose

   Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   CAG-LSL-GCaMP6-fast
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. STARR-seq luciferase validation vector_ORI_AGAP1

   Plasmid

   #99300

   Purpose

   Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr2: 236735694-236737193 (AGAP1 Human)
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. STARR-seq luciferase validation vector_mP_AGAP1

   Plasmid

   #99301

   Purpose

   Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr2: 236735694-236737193 (AGAP1 Human)
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. STARR-seq luciferase validation vector_SCP1_AGAP1

   Plasmid

   #99302

   Purpose

   Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr2: 236735694-236737193 (AGAP1 Human)
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Snap25-LSL-2A-GFP targeting vector

   Plasmid

   #61575

   Purpose

   Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Snap25-LSL-2A-GFP
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

    Plasmid

    #73707

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    T231A/S232A

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

11. HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector

    Plasmid

    #73706

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451 and D143N

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Rosa26-LSL-PE2 Targeting Vector

    Plasmid

    #196038

    Purpose

    The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Insert

    Cas9-MMLV-RT
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV PIG (Puro IRES GFP empty vector)

    Plasmid

    #21654

    Purpose

    Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+) CircRNA Mini Vector-ZKSCAN1 nt 548-1047

    Plasmid

    #60649

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Calb1-T2A-dgCre targeting vector

    Plasmid

    #114438

    Purpose

    Target dgCre into the endogenous mouse Calb1 locus at the stop codon

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    dgCre
    

    Use

    Mouse Targeting

    Tags

    DHFR domain and EGFP

    Expression

    Mutation

    Promoter

    none; utilizes endogenous Calb1 promoter for expr…

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid

    #132962

    Purpose

    Firefly-luciferase single-strand annealing assay cloning backbone

    Depositor

    Gang Bao

    Article

    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

    Insert

    Firefly Luciferase
    

    Use

    CRISPR and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Stop sequence between luciferase gene

    Promoter

    CMV

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-FHH-Empty Vector-IRES-Puro

    Plasmid

    #120568

    Purpose

    For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Flag-HA-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac His6 MBP N10 TEV LIC cloning vector (4C)

    Plasmid

    #30116

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

    Plasmid

    #55215

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    2xStrep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)

    Plasmid

    #29725

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), SiriusGFP, and TEV cleava…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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