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pSMART-HCKan-yibDp-VHH-3E3

Plasmid

#202474

Purpose

Expresses the nanobody 3E3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 3E3

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-C9BoNT-A

Plasmid

#202475

Purpose

Expresses the nanobody C9 BoNT-A after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-E7BoNT-A

Plasmid

#202476

Purpose

Expresses the nanobody E7 BoNT-A after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody E7 BoNT/A

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-JM3

Plasmid

#202477

Purpose

Expresses the nanobody JM3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody JM3

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-J3

Plasmid

#202478

Purpose

Expresses the nanobody J3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody J3

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-m36.4

Plasmid

#202479

Purpose

Expresses the nanobody m36.4 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody m36.4

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-Re5D06

Plasmid

#202480

Purpose

Expresses the nanobody Re5D06 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody Re5D06

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-Re9F06

Plasmid

#202481

Purpose

Expresses the nanobody Re9F06 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody Re9F06

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-ppiB

Plasmid

#202482

Purpose

Expresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

peptidyl-prolyl cis-trans isomerase (ppiB)

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCASCADE-LCAmp-trxB

Plasmid

#202465

Purpose

Expresses a guide RNA to silence trxB after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

trxB guide RNA

Expression

Bacterial

Promoter

ugpB

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLT237

Plasmid

#174300

Purpose

Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

PolC homology arm

Expression

Bacterial

Mutation

changed Cysteine 669 to Tyrosine

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDF-pntAB

Plasmid

#158609

Purpose

Low phosphate induction of pntAB

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)

Expression

Bacterial

Promoter

Insulated ugpBp from E. coli

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHCKan-ydfHp-GFPuv

Plasmid

#139079

Purpose

Low phosphate inducible promoter ydfHp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

ydfHp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phnCp-GFPuv

Plasmid

#139080

Purpose

Low phosphate inducible promoter phnCp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phnCp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-tktBp-GFPuv

Plasmid

#139081

Purpose

Low phosphate inducible promoter tktBp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

tktBp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-asrp-GFPuv

Plasmid

#139082

Purpose

Low phosphate inducible promoter asrp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

asrp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yegHp-GFPuv

Plasmid

#139084

Purpose

Low phosphate inducible promoter yegHp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

yegHp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-ytfKp-GFPuv

Plasmid

#139085

Purpose

Low phosphate inducible promoter ytfKp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

ytfKp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-amnp-GFPuv

Plasmid

#139070

Purpose

Low phosphate inducible promoter amnp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

amnp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-mipAp-GFPuv

Plasmid

#139071

Purpose

Low phosphate inducible promoter mipAp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

mipAp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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