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Plasmid#149277PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNLS_P2A_Hygro_Barcode
Plasmid#120506PurposeBarcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC H84NLS (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of nuclear localization signal sequencePromoterEF1aAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-1-RBD-8h
Plasmid#161821PurposeMammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)DepositorInsertReceptor-binding domain (RBD) of spike protein S
UseTagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T320-D518)PromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-BNT162b2ΔUTR
Plasmid#171214PurposeGateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.DepositorInsertBNT162b2ΔUTR
UseGateway cloningTagsExpressionMutationKozak sequence changed from CCCGCCACC to GGCTGCAC…PromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)
Plasmid#149278PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN6kwh
Plasmid#44724DepositorInsertspCMV-D2i promoter
htetR::NLS
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6MBW6
Plasmid#103102PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-G2ZYP2
Plasmid#103110PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertG2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q927P4
Plasmid#103131PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsGIGYF2_AE
Plasmid#148550PurposeMammalian Expression of HsGIGYF2DepositorInsertHsGIGYF2 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLB2 5′ mouse CNS
Plasmid#49348Purposeluciferase reporter with mouse Bmp2 promoter and 3' conserved noncoding sequence (CNS)DepositorUseLuciferaseTagsluciferaseExpressionMutationPromoter-Available sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_AE
Plasmid#148577PurposeMammalian Expression of HsGIGYF2DepositorInsertHsGIGYF2 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-B2UP49_GFP
Plasmid#103071PurposeCas9 coding gene template with optimized sequence for human codon usage, also expresses EGFPDepositorInsertB2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
UseCRISPRTagsExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A0LWB3
Plasmid#103070PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-K1LM04
Plasmid#103119PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6I4Q5
Plasmid#103101PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F3UXG6
Plasmid#103108PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-C7TEQ6
Plasmid#103092PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertC7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL
Plasmid#58414PurposeExpresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHA internalExpressionMammalianMutationCysteines 71, 72, and 105 to Alanine; The sequenc…PromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only