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We narrowed to 11,198 results for: CHL

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Showing: 2981 - 3000 of 11198 results

  3. pSicoR-Ef1a-mCh-Puro

    Plasmid
    #31845
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Promoter
    U6 for shRNA; Ef1alpha for mCherry-puromycin
    Available Since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31845-plasmid-map-sequence-id-194345

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  6. pTriEx-PA-Rac1

    Plasmid
    #22024
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  9. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid
    #208963
    Purpose
    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    EcKlenow-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208963-plasmid-map-sequence-id-415798

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  10. pTP434

    Plasmid
    #104167
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling
    Depositor
    Dirk Görlich
    Article
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    3x Cysteines located at bps 493-495, 520-522, and…
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104167-plasmid-map-sequence-id-200061

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  11. pKK-TEV-CyOFP1

    Plasmid
    #105799
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-CyOFP1
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105799-plasmid-map-sequence-id-203392

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  13. pET30a-His6-ABP-LOXCAT

    Plasmid
    #139971
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139971-plasmid-map-sequence-id-311161

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  14. pTriEx-mVenus-PA-Rac1

    Plasmid
    #22007
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

    Plasmid
    #114377
    Purpose
    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting
    Depositor
    Mingshan Xue
    Article
    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10
    Insert
    GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
    Use
    AAV
    Tags
    EYFP and Kv2.1C-linker-TlcnC
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114377-plasmid-map-sequence-id-220125

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  17. PB-CAG-ChR2-YFP

    Plasmid
    #204345
    Purpose
    PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.
    Depositor
    Ivo Lieberam
    Article
    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi
    Insert
    ChR2-YFP
    Tags
    Yellow fluorescent protein
    Expression
    Mammalian
    Mutation
    C-terminus (aa315-737) is deleted - PMID: 1461559…
    Promoter
    CAG
    Available Since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    204345-plasmid-map-sequence-id-410725

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  19. TFORF1803

    Plasmid
    #143318
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    USF1 (USF1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143318-plasmid-map-sequence-id-408542

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  20. pKK-CyOFP1-TEV

    Plasmid
    #105782
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    CyOFP1-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105782-plasmid-map-sequence-id-203371

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Showing: 2981 - 3000 of 11198 results