We narrowed to 6,115 results for: cat.2

1. pcDNA3-Tet2

   Plasmid

   #60939

   Purpose

   Expresses Tet2 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 2 (Tet2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET His6 LIC cloning vector (2Bc-T)

   Plasmid

   #37236

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. VE-cad-eGFP Donor plasmid

   Plasmid

   #92309

   Purpose

   Knock eGFP into human VE-cad locus

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad Homologous Recombination Arms (CDH5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   about 30 a.a. truncated at end of protein

   Promoter

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. VE-cad KI gRNA1

   Plasmid

   #92310

   Purpose

   CRISPR-GFP-gRNA for cutting VEcad

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad KI gRNA1 (CDH5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMT3-FBF2 AS/Y

   Plasmid

   #166974

   Purpose

   Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575…

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-N-Myc_C223S USP7

   Plasmid

   #131243

   Purpose

   Mammalian expression of catalytically inactive Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 cyst…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPO5

   Plasmid

   #209987

   Purpose

   Contains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   replication origin (256_rep)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1‐Afu‐rnhB D101N

   Plasmid

   #108695

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D101N catalytic site mutation

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATeam1.03-nD/nA/pcDNA3

   Plasmid

   #51958

   Purpose

   mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

   Depositor

   Takeharu Nagai

   Article

   Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

   Insert

   ATeam1.03-nD/nA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ateam1.03-nD/nA/pRSETB

    Plasmid

    #51960

    Purpose

    bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ateam1.03-nD/nA/pENTR1A

    Plasmid

    #51959

    Purpose

    Gateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. RCAS GFP (CT#22)

    Plasmid

    #13878

    Depositor

    Connie Cepko

    Article

    Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

    Insert

    GFP
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1(+) eGFP p6

    Plasmid

    #78582

    Purpose

    Expresses GFP p6 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Gurer et al J Virol. 2005 Jan;79(2):910-7.

    Insert

    eGFP p6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HIV-1 HXB2 p6 with conservative mutations (G78A, …

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRIAS (CC#15)

    Plasmid

    #15147

    Depositor

    Connie Cepko

    Article

    Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

    Type

    Empty backbone

    Use

    Retroviral; Avian expression

    Tags

    gag and pol

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIRES-11 (CC#13)

    Plasmid

    #15145

    Depositor

    Connie Cepko

    Article

    Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

    Type

    Empty backbone

    Use

    Adapter vector for cloning into prisap

    Tags

    IRES

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRU5HT1-inLuc

    Plasmid

    #58954

    Purpose

    HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

    Depositor

    Dmitriy Mazurov, David Derse

    Article

    Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

    Insert

    inLuc
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CMV/R/U5

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111926

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111928

    Purpose

    Orange color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111927

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CeNL(Ca2+)_110μ-pcDNA3

    Plasmid

    #111929

    Purpose

    Cyan color bright luminescent calcium indicator with low affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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