We narrowed to 3,399 results for: guide rna expression plasmid

1. AiP939-pX330-Ctxn3

   Plasmid

   #181769

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Ctxn3 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS402-GPD-GFP(S65T)

   Plasmid

   #85190

   Purpose

   Integrating plasmid, GFP(S65T) under GPD promoter

   Depositor

   David Bartel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   EGFP
   

   Use

   Integrating, shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Dec. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH1v1

   Plasmid

   #60244

   Purpose

   gRNA expression by the H1 promoter

   Depositor

   Donald Zack

   Article

   Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 promoter

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB70

   Plasmid

   #47943

   Purpose

   Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

   Plasmid

   #83306

   Purpose

   Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

   Depositor

   Tohru Kimura

   Article

   Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

   Insert

   sgRNA and dCas9 from pX330
   

   Use

   Lentiviral

   Tags

   FLAG and PA tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA and CBh for dCas9

   Available since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT040

   Plasmid

   #67640

   Purpose

   URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Insert

   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-LambdaN-NbALFA

   Plasmid

   #207627

   Purpose

   Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   LambdaN-NbALFA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pCMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR::rpsL

   Plasmid

   #44505

   Purpose

   A crRNA expression plasmid specific to the rpsL allele.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR::rpsL (rpsL )
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS405-LEU2-TEF-KpolDcr1v2

   Plasmid

   #85193

   Purpose

   Integrating plasmid, K. polysporus Dcr1 under TEF promoter

   Depositor

   David Bartel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   Dcr1
   

   Use

   Integrating, shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSliceRep_B_miR-196a.M3

    Plasmid

    #220471

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSliceRep_A_miR-196a.M3

    Plasmid

    #220467

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSliceRep_B_miR-7

    Plasmid

    #220468

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSliceRep_B_miR-196a

    Plasmid

    #220470

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSliceRep_B_miR-7.M4

    Plasmid

    #220469

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    SUMO_Eu1
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSliceRep_A_miR-7

    Plasmid

    #220464

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSliceRep_A_miR-7.M4

    Plasmid

    #220465

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSliceRep_A_miR-196a

    Plasmid

    #220466

    Purpose

    Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Superfolder GFP
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC155-pCR8-sgExpression

    Plasmid

    #49045

    Purpose

    U6-driven sgRNA expressing cassette on a gateway donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC119-MCS

    Plasmid

    #58807

    Purpose

    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.

    Depositor

    Jen Sheen

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRGEB33

    Plasmid

    #158151

    Purpose

    Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge