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C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
nTALE+CD_for TUB_8N_1397NC

Plasmid

#193383

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For multiplex C-to-T base editing to introduce amino acid mutations in the coding sequence of AtTUBs.

Expression

Plant

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn-PdCO-mScarlet-WPRE

Plasmid

#198510

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

Plasmid

#198508

Purpose

Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

CaMKIIα minimal promotor (0.4kb)

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn-DIO-PdCO-mScarlet-WPRE

Plasmid

#198511

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

Plasmid

#198516

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

Plasmid

#198514

Purpose

Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CaMKIIα minimal promotor (0.4kb)

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn-PdCO-EGFP-WPRE

Plasmid

#198513

Purpose

Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SIO-PdCO-mScarlet-WPRE

Plasmid

#198509

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

Plasmid

#198515

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 delta e4-T2A-GFP

Plasmid

#163771

Purpose

Expression of CARD domain variant with a deletion of exon 4

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

Del exon 4

Promoter

MND

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 delta e3-4-T2A-GFP

Plasmid

#163772

Purpose

Expression of CARD domain variant with a deletion of exons 3 and 4.

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

Del exon 3-4

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 delta e3-T2A-GFP

Plasmid

#163770

Purpose

Expression of CARD domain variant with a deletion of exon 3

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

Del exon 3

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EcNR1 Strain

Bacterial Strain

#26930

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Ampicillin

Available Since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
pre-associated oBC-mBC backbone (p025)

Pooled Library

#194097

Purpose

pre-associated oBC-mBC backbone (p025), can insert additional reporter constructs

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
scQers promoter control #2 (low: no promoter, p029)

Pooled Library

#217421

Purpose

scQers promoter control #2 (low: no promoter, p029)

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Expression

Plant

Promoter

RPS5A

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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OA-16

Plasmid

#104966

Purpose

Plasmid expressing two multiplexed ribozyme-gRNA-ribozyme (RGR) gRNAs

Depositor

Omar Akbari

Article

Marshall et al Sci Rep. 2017 Jun 19;7(1):3776. doi: 10.

Insert

Ribozyme-gRNA-ribozyme

Use

Cre/Lox

Expression

Insect

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

Plasmid

#202199

Purpose

Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

stChrimsonR, PdCO

Use

AAV and Cre/Lox

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

Plasmid

#202198

Purpose

Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

stChrimsonR, PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CaMKIIα minimal promotor (0.4kb)

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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