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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,460 results for: spl

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Showing: 3021 - 3040 of 8460 results

  5. pUC19-Trigger-Bottom

    Plasmid
    #87317
    Purpose
    Expresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.
    Depositor
    Donald Burke
    Publication
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli "Bottom" transcript downstream of RNA Trigger (TS2_AT01)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    P70a
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87317-plasmid-map-sequence-id-174671

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  6. pUC19-Trigger-Bottom

    Plasmid
    #87317
    Purpose
    Expresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.
    Depositor
    Donald Burke
    Publication
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli "Bottom" transcript downstream of RNA Trigger (TS2_AT01)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    P70a
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87317-plasmid-map-sequence-id-174671

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  11. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid
    #232344
    Purpose
    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag
    Depositor
    Philip Wong
    Publication
    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HDGF Like 2 (HDGFL2 Human)
    Tags
    GGGS linker, 6xHis and Twin-strep, TEV protease s…
    Expression
    Mammalian
    Mutation
    TDP-43 regulated cryptic exon
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232344-plasmid-map-sequence-id-462328

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  12. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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  13. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid
    #59826
    Purpose
    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.
    Depositor
    Stephen Taylor
    Publication
    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Separase (ESPL1 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…
    Promoter
    hybrid human cytomegalovirus (CMV)/TetO2 promoter
    Available since
    Feb. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    59826-plasmid-map-sequence-id-99124

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  15. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid
    #232344
    Purpose
    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag
    Depositor
    Philip Wong
    Publication
    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HDGF Like 2 (HDGFL2 Human)
    Tags
    GGGS linker, 6xHis and Twin-strep, TEV protease s…
    Expression
    Mammalian
    Mutation
    TDP-43 regulated cryptic exon
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232344-plasmid-map-sequence-id-462328

    Enlarge

  16. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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  17. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid
    #59826
    Purpose
    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.
    Depositor
    Stephen Taylor
    Publication
    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Separase (ESPL1 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…
    Promoter
    hybrid human cytomegalovirus (CMV)/TetO2 promoter
    Available since
    Feb. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    59826-plasmid-map-sequence-id-99124

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  20. pJD427

    Plasmid
    #154371
    Purpose
    Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters
    Depositor
    Joseph Davis
    Publication
    Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13
    Insert
    proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
    Expression
    Bacterial
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    154371-plasmid-map-sequence-id-311827

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Showing: 3021 - 3040 of 8460 results