We narrowed to 4,430 results for: Erf

1. PB CAG-L-bpA-SV40pA-L-td-sfGFP

   Plasmid

   #133575

   Purpose

   CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Tandem dimer superfolder GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPD2 dCas9 (GB2664)

   Plasmid

   #160596

   Purpose

   CDS of dCas9 with B3 nomenclature

   Depositor

   Diego Orzaez

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB CAG-R-bpA-2xSV40pA-R-td-sfGFP

   Plasmid

   #133578

   Purpose

   CAG tdGFP dre/rox reporter piggyBac transposon (bpA 2x SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Tandem dimer superfolder GFP
   

   Use

   Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB CAG-F-bpA-SV40pA-F-td-sfGFP

   Plasmid

   #133571

   Purpose

   CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 1x SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Tandem dimer superfolder GFP
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. C100m

   Plasmid

   #121004

   Purpose

   MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   sfYFP with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. C41m

   Plasmid

   #120974

   Purpose

   MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR Ef1a-dCas9-BFP-KRAB

   Plasmid

   #217304

   Purpose

   lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

   Depositor

   Luke Gilbert

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExp-sfGFP

   Plasmid

   #112573

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis, Strep II, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-dCas9-KRAB_Hygro

   Plasmid

   #192663

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH385-GST-TEV-SUP35

    Plasmid

    #29744

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    Available since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH278-GST-TEV-SUP45

    Plasmid

    #29743

    Depositor

    Tobias von der Haar

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRL-TK CXCR4 1P

    Plasmid

    #11309

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    1 perfect bind site for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Rr-luc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB CAG-R-3xSV40pA-R-td-sfGFP

    Plasmid

    #177150

    Purpose

    CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Dre / rox ; piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3 CXCR4 1P

    Plasmid

    #11310

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    1 perfect binding site for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-loxP-3xpA-H2BsfGFP-tdTomato - opt GS PQR Var3 P2A -APGST E

    Plasmid

    #113847

    Purpose

    Histone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-P2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3 CXCR4 2P

    Plasmid

    #11311

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    2 perfect binding sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. U6+27-16pf

    Plasmid

    #21976

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-16 perfect
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pTH340-SUP45(1-242)-GalBD

    Plasmid

    #29740

    Depositor

    Tobias von der Haar

    Insert

    SUP45 (SUP45 Budding Yeast)
    

    Use

    Tags

    Gal Activation domain and Myc

    Expression

    Yeast

    Mutation

    Codons 1-242 of the yeast SUP45 gene only

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3 CXCR4 3P

    Plasmid

    #11312

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    3 perfect binding sites for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB CAG-R-bpA-3xSV40pA-R-td-sfGFP

    Plasmid

    #133579

    Purpose

    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge