We narrowed to 3,233 results for: multiplex

1. EcNGRfEfC-fadD-tesA'-fadR

   Bacterial Strain

   #48939

   Purpose

   Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)

   Depositor

   George Church

   Bacterial Resistance

   Spectinomycin

   Available since

   Dec. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pSH-Csy4-T2A-SpRFN

   Plasmid

   #85754

   Purpose

   Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-dCas9
   

   Use

   CRISPR

   Tags

   Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSH-Csy4-T2A-SaRFN

   Plasmid

   #85755

   Purpose

   Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-SadCas9
   

   Use

   CRISPR

   Tags

   3xHA tag, Csy4, and FokI fusion via 36 amino acid…

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CAG

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCHKO_TK1_HPRT1_Lb_4

   Plasmid

   #155062

   Purpose

   Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLCHKO_TK1_HPRT1_Lb_6

   Plasmid

   #155064

   Purpose

   Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLCHKO_TK1_HPRT1_Lb_5

   Plasmid

   #155063

   Purpose

   Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLCHKO_TK1_HPRT1_Lb_3

   Plasmid

   #155061

   Purpose

   Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLCHKO_TK1_HPRT1_Lb_2

   Plasmid

   #155060

   Purpose

   Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

   Plasmid

   #85756

   Purpose

   All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Inserts

   Csy4-T2A-SpRFN-P2A-GFP
   gRNA
   

   Use

   CRISPR

   Tags

   Csy4, Csy4 recognition sequence, FokI fusion via …

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CMV and U6

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR3_BC2

    Plasmid

    #191857

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPR3_BC6

    Plasmid

    #191861

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPR3_BC5

    Plasmid

    #191860

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPR3_BC4

    Plasmid

    #191859

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR3_BC3

    Plasmid

    #191858

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR3_BC1

    Plasmid

    #191856

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AiP14460: pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BarcodeR-BGHpA (Alias: CN4460)

    Plasmid

    #236599

    Purpose

    Barcoded enhancer AAV vector for multiplexed screening applications; AiE0452h_3xC2 is an enhancer active in striatal D2-MSNs and cortical L5_ET neurons

    Depositor

    Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AiP14454: pAAV-AiE0783m-minBG-SYFP2-WPRE3-BarcodeL-BGHpA (Alias: CN4454)

    Plasmid

    #236593

    Purpose

    Barcoded enhancer AAV vector for multiplexed screening applications; AiE0783m is an enhancer active in striatal MSNs

    Depositor

    Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AiP14453: pAAV-AiE0441h-minBG-SYFP2-WPRE3-BarcodeK-BGHpA (Alias: CN4453)

    Plasmid

    #236592

    Purpose

    Barcoded enhancer AAV vector for multiplexed screening applications; AiE0441h is an enhancer active in striatal MSNs

    Depositor

    Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AiP14449: pAAV-AiE0367h_3xC2-minRho-SYFP2-WPRE3-BarcodeG-BGHpA (Alias: CN4449)

    Plasmid

    #236588

    Purpose

    Barcoded enhancer AAV vector for multiplexed screening applications; AiE0367h_3xC2 is an enhancer active in striatal MSNs

    Depositor

    Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP14461: pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BarcodeS-BGHpA (Alias: CN4461)

    Plasmid

    #236600

    Purpose

    Barcoded enhancer AAV vector for multiplexed screening applications; AiE0779m_3xC2 is an enhancer active in striatal D1-MSNs

    Depositor

    Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge