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pCMV-Nxph4-4Q-HA

Plasmid

#128104

Purpose

Express HA tagged Nxph4-4Q

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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mOrange2-Alpha-Actinin-19

Plasmid

#57944

Purpose

Localization: Alpha Actinin, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pPK700

Plasmid

#38146

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

let-60p::ptc-3::gfp (ptc-3 Nematode)

Tags

EGFP

Expression

Worm

Promoter

let-60

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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TPOR-SmBiT Vector

Plasmid

#238780

Purpose

Express TPOR-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

TPOR (MPL Human)

Tags

SmBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pUC18 HA PTPL1

Plasmid

#237335

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

PTPL1 (PTPN13 Human)

Tags

HA

Expression

Bacterial

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-TASL-NEMO

Plasmid

#221267

Purpose

Expression of TASL-NEMO IKK binding site in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

fusion of TASL with NEMO IKK binding site (Tasl, Ikbkg Mouse)

Use

Retroviral

Tags

3xFLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-iStat3-BFP

Plasmid

#216870

Purpose

Lenti-iStat3-BFP plasmid contains the TRE (doxycycline inducible) promoter followed by the Stat3 mouse cDNA, a T2A and a Blue Fluorescent Protein (BFP) sequence.

Depositor

Bernhard Payer

Article

Barrero et al Sci Adv. 2024 Aug 9;10(32):eadj8862. doi

Insert

Stat3 (Stat3 Mouse)

Use

Lentiviral

Tags

T2A-TagBFP

Expression

Mammalian

Promoter

Tetracycline-Response-Element (TRE)

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.1

Plasmid

#208302

Purpose

Expresses ABE-RA3.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.1-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#2/Cre

Plasmid

#173648

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#1/Cre

Plasmid

#173647

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmem53-V5

Plasmid

#175142

Purpose

Lentiviral expression of mouse Tmem53-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem53 (Tmem53 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A_mmFbxw7(res) (closed)

Plasmid

#160105

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway Cloning

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

Plasmid

#136651

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9:CNRZ_1066 Chimera
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG and hU6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-Reverse-Complement

Plasmid

#136016

Purpose

The Reverse Complement of EIF3B 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (Reverse Complement) (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

Plasmid

#132686

Purpose

AAV plasmid encoding mouse ACY1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Acylase I (Acy1, Acy1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-T1025A

Plasmid

#116543

Purpose

Lentiviral expression of PIK3CA T1025A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

T1025A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-V344M

Plasmid

#116548

Purpose

Lentiviral expression of PIK3CA V344M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

V344M

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-Y1021C

Plasmid

#116549

Purpose

Lentiviral expression of PIK3CA Y1021C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

Y1021C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-R38C

Plasmid

#116535

Purpose

Lentiviral expression of PIK3CA R38C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

R38C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-R93P

Plasmid

#116539

Purpose

Lentiviral expression of PIK3CA R93P

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

R93P

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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