We narrowed to 29,578 results for: PLE

1. pUC57-RPLP15UTR-hRluc-A60

   Plasmid

   #182639

   Purpose

   Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

   Depositor

   Stephen Floor

   Article

   Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

   Insert

   Renilla luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   The transcription is driven by a T7 promoter, pai…

   Promoter

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIG1_UL136_19kDa_EE

   Plasmid

   #74951

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Glu-Glu tag (EYMPME)

   Expression

   Mammalian

   Mutation

   delta 1-127

   Promoter

   CMV, lentiviral LTR

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIG1_UL136_23kDa_Myc

   Plasmid

   #74949

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   HA Tag (YPYDVPDYA)

   Expression

   Mammalian

   Mutation

   delta 1-99 M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS1957

   Plasmid

   #82568

   Purpose

   High copy number plasmid containing hs1218 MiniPromoter insert.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   hshs1218
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEMS1953

    Plasmid

    #82567

    Purpose

    High copy number plasmid containing hs671 MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    hs671
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pCIG1_UL136_25kDa_Myc

    Plasmid

    #74954

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

    Expression

    Mammalian

    Mutation

    delta 1-77, M100A M128A

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

    Plasmid

    #61796

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Ywhab 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

    Plasmid

    #61793

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Xbp1 3'UTR with miR-200c MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

    Plasmid

    #61791

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad5 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

15. psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

    Plasmid

    #61790

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Map3k1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

16. psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

    Plasmid

    #61789

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    both miR-200c MRE-2 and MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

17. psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

    Plasmid

    #61786

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Crtap 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.2-R5A-K6A C11orf83-V5

    Plasmid

    #65850

    Purpose

    Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6A

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Mutated UQCC3 (UQCC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R5A-K6A

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.2-R5D-K6D C11orf83-V5

    Plasmid

    #65851

    Purpose

    Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6D

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Mutated UQCC3 (UQCC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R5D-K6D

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

    Plasmid

    #61787

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Fhod1 3'UTR with miR-200c MRE-2 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-2 mutated

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only