We narrowed to 5,570 results for: donor

1. AICSDP-13:FBL-mEGFP

   Plasmid

   #87427

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   FBL Homology Arms with linker-mEGFP (FBL Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pORANGE GFP-CACNA1A KI

   Plasmid

   #131480

   Purpose

   Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE mEos3.2-Camk2a KI

   Plasmid

   #131493

   Purpose

   Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and mEos3.2 donor (Camk2a, Camk2a Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-46: MYL7-mEGFP

   Plasmid

   #114413

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

   Plasmid

   #216320

   Purpose

   Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Luciferase + splice donor site
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-ZNF736

   Plasmid

   #101602

   Purpose

   Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF736 (ZNF736 Human)
   

   Use

   Gateway shuttling vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Control-cx43.4-48H-pGTag

   Plasmid

   #117818

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   CAAX

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUt-mPF-GFP

   Plasmid

   #199222

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   rtTA-Advanced::P2A::EGFP
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICOZ-SB100X[EF1α-CD19 4-1BB]

   Plasmid

   #218328

   Purpose

   Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   CD19 CAR mammalian expression cassette
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE GFP-Arpc5 KI

    Plasmid

    #131503

    Purpose

    Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Arpc5, Arpc5 Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR2.1-KRAS-G12V

    Plasmid

    #129091

    Purpose

    KI KRAS G12V mutation into human KRAS locus

    Depositor

    Toshiro Sato

    Article

    Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

    Insert

    KRAS(G12V) (KRAS Human)
    

    Use

    Homologous recombination donor plasmid

    Mutation

    G12V

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQLL-Tc1-1_Xt[EF1α-copGFP]

    Plasmid

    #218325

    Purpose

    Donor plasmid of Tc1-1_Xt Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMV-Tc1-2_ST

    Plasmid

    #217289

    Purpose

    Donor plasmid of Tc1-2_ST Transposon

    Depositor

    Yong E Zhang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    TIRs of Tc1-2_ST transposon
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMV-piggyBac-1_AMi

    Plasmid

    #217236

    Purpose

    Donor plasmid of piggyBac-1_AMi Transposon

    Depositor

    Yong E Zhang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    TIRs of piggyBac-1_AMi transposon
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE GFP-CACNA1E KI

    Plasmid

    #131481

    Purpose

    Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1e, Cacna1e Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR-53BP1 1-1972 28A

    Plasmid

    #53447

    Purpose

    Recombinational donor/master vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Gateway

    Mutation

    I914T mutation; All 28 SQ/TQ motifs in N-term of …

    Available Since

    Dec. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pPRISM-Cre-cmlc2-eGFP

    Plasmid

    #117794

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    Cre
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-ZNF251

    Plasmid

    #101451

    Purpose

    Donor Vector containing ZNF251 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF251 (ZNF251 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid

    #119189

    Purpose

    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.

    Depositor

    Alex Dunn

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    

    Use

    Transposon

    Tags

    mTFP1-F40-mEYFP

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    TCE

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQLL-Tc1-8B_DR[EF1α-copGFP]

    Plasmid

    #218327

    Purpose

    Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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