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pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

Plasmid

#85434

Purpose

Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab

Use

Lentiviral

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDN34

Plasmid

#61343

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Publication

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS2 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pZW1-FCS-circRNA

Plasmid

#73449

Purpose

Expression of circular RNA

Depositor

Ling-Ling Chen

Publication

Zhang et al Cell. 2014 Sep 25;159(1):134-47. doi: 10

Insert

POLR2A (POLR2A Human)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

CMV promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLA230

Plasmid

#11724

Depositor

Lawrence Loeb

Publication

Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

Insert

b-lactamase

Expression

Bacterial

Mutation

GAA for Glu26 changed to TAA (premature stop codo…

Available since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pUCBB-pT7-eGFP

Plasmid

#32552

Depositor

Claudia Schmidt-dannert

Publication

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

pT7

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13

Plasmid

#13559

Depositor

Wolf Frommer

Publication

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Delta13

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV_psychLight2

Plasmid

#163910

Purpose

Expresses psychLight2 in mammalian cells

Depositor

Lin Tian

Publication

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Insert

psychLight2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMOD_A0501

Plasmid

#91011

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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911 pBabe puroL PTEN G129E

Plasmid

#10771

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Use

Retroviral

Expression

Mammalian

Mutation

G129E

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCDNA3.1(+)-NEMOc

Plasmid

#189933

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Publication

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOc

Expression

Mammalian

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry

Plasmid

#85430

Purpose

[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-10 T2A PD-L1

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pS3

Plasmid

#50596

Purpose

Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

Depositor

Jay Keasling

Publication

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Inserts

aroE
aroD
aroB
aroG*
ppsA
tktA

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCEV-G4-Km

Plasmid

#46819

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pX330S-5

Plasmid

#58781

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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4xHRE_YB TATA-sfGFP-CMV_dsRed

Plasmid

#117399

Purpose

sfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Publication

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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863 pSG5L HA NES PTEN

Plasmid

#10932

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

HA and NES

Expression

Mammalian

Available since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDIRECT_25F

Plasmid

#91143

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA

Use

CRISPR

Expression

Plant

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A-1x4

Plasmid

#58768

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pM-CBE

Plasmid

#196293

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding CBE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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