We narrowed to 23,022 results for: form

1. pLB(N)CX AT-CH3-FLAG-HA WY

   Plasmid

   #133725

   Purpose

   Retroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).

   Depositor

   James A. DeCaprio

   Article

   Borger et al J Virol. 2006 May . 80(9):4292-303.

   Insert

   EP300 (EP300 Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Mutation

   partial (not complete) p300 fragment, AT and CH3 …

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-Hemmar-DSCP-ftz-FLP(D)

   Plasmid

   #119894

   Purpose

   FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

   Depositor

   Rita Sousa-Nunes

   Article

   Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

   Inserts

   FLP(D)
   DSCP-ftz (ftz Fly)
   

   Use

   Drosophila expression

   Mutation

   a mutated form of FLP, which at position 5 contai…

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-beta2(616-951)Y815A

   Plasmid

   #100744

   Purpose

   Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Long isoform with Y815A mutation

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

   Plasmid

   #80325

   Purpose

   Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

   Depositor

   Daniel Schoenberg

   Article

   Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

   Insert

   PXDNL-003 (PXDNL Human)
   

   Tags

   Biotin

   Expression

   Mammalian

   Mutation

   H170A and H256A

   Promoter

   CMV

   Available Since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. NET39 Short pmRFP-N2 (451)

   Plasmid

   #61981

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET39 (PPAPDC3 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Short form of NET39

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. YCpADE1

   Plasmid

   #22494

   Depositor

   Beth Montelone

   Insert

   ADE1 (ADE1 Budding Yeast)
   

   Use

   Yeast shuttle

   Available Since

   Dec. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBac[3xP3 g Tc' v]

   Plasmid

   #86446

   Purpose

   pBAc transposon with transformation marker for Tribolium transgenesis; Rescues vermilion white eyed mutant to black eyes; (transformation plasmid)

   Depositor

   Gregor Bucher

   Insert

   3xP3 g Tc' v
   

   Expression

   Insect

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK515

   Plasmid

   #72385

   Purpose

   Produces Escherichia coli glycinamide ribonucleotide formyltransferase 2 (EcPurT)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylglycinamide formyltransferase 2
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MT1K_K20T

    Plasmid

    #105708

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1k subisoform with Thr at positon 20

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1k
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    This subisoform has a K20T substitution

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIMAY

    Plasmid

    #68939

    Purpose

    E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

    Depositor

    Tim Foster

    Article

    Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

    Type

    Empty backbone

    Use

    Staphylococcal allelic exchange vector

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK556

    Plasmid

    #74277

    Purpose

    Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes alanine-1246 to serine, numerous silent m…

    Promoter

    T7

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIKM1

    Plasmid

    #51054

    Purpose

    Kanamycin and erethromycin resistance

    Depositor

    Juergen Wiegel, Lee Lynd

    Article

    Tyurin et al Appl Environ Microbiol. 2004 Feb;70(2):8

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. FmnI IS04 cDNA/pcDNA3

    Plasmid

    #19321

    Depositor

    Philip Leder

    Article

    Dettenhofer et al PLoS ONE. 2008 . 3(6):e2497.

    Insert

    FmnI Iso4 cDNA (Fmn1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pET29b-pvdF WT

    Plasmid

    #187974

    Purpose

    Expresses hyrdoxyornithine transformylase PvdF from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Kenjic et al Arch Biochem Biophys. 2019 Mar 30;664:40

    Insert

    pvdF WT
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK040 (pET-PurQ-A128T)

    Plasmid

    #73840

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Expression

    Bacterial

    Mutation

    A128T mutant

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK044 (pET-PurQ-wt)

    Plasmid

    #73839

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBHt2G-RFP

    Plasmid

    #107162

    Purpose

    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

    Depositor

    Sophien Kamoun

    Type

    Empty backbone

    Use

    Fungal grobacterium transformation plasmid, teste…

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK539

    Plasmid

    #72515

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK549

    Plasmid

    #72520

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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