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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 598 results for: SPY

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Showing: 301 - 320 of 598 results

  6. pICSL50051

    Plasmid
    #245607
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag 3xFLAG-TEV-Spy affinity peptide
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245607-plasmid-map-sequence-id-495580

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  7. pICSL30040

    Plasmid
    #245545
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Spy affinity peptide-TEV-Myc
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245545-plasmid-map-sequence-id-495473

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  8. pICSL30041

    Plasmid
    #245546
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Spy affinity peptide-C3-Myc
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245546-plasmid-map-sequence-id-495474

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  9. pICSL30065

    Plasmid
    #245568
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving SPY tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245568-plasmid-map-sequence-id-495492

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  10. SUMO-KTag

    Plasmid
    #51723
    Purpose
    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SUMO-KTag
    Tags
    His6 Tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pSECRETS-B

    Plasmid
    #196987
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    Spy gRNA cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pltetO1
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196987-plasmid-map-sequence-id-389302

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Showing: 301 - 320 of 598 results