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We narrowed to 388 results for: SaCas9

Showing: 301 - 320 of 388 results

  1. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Article
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    96921-plasmid-map-sequence-id-181715

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  4. AIDx-ID-1058

    Plasmid
    #135347
    Purpose
    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus
    Depositor
    Alex Hewitt
    Article
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Insert
    nSpyCas9-hAIDx-ID-1058-UGI
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135347-plasmid-map-sequence-id-267696

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  11. S1 1029 CP ABEMax strategy 2

    Plasmid
    #135358
    Purpose
    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)
    Depositor
    Alex Hewitt
    Article
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Insert
    WT Tada-nSpyCas9 aa 1029-evolved Tada
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135358-plasmid-map-sequence-id-267714

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  15. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  16. PDG458 eSp(1.1) V3

    Plasmid
    #226964
    Purpose
    CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226964-plasmid-map-sequence-id-450825

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  17. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  18. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

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  19. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

    Plasmid
    #240865
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240865-plasmid-map-sequence-id-482051

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  20. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

    Plasmid
    #240094
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240094-plasmid-map-sequence-id-482052

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Showing: 301 - 320 of 388 results