Addgene

We narrowed to 15,118 results for: nans

Showing: 301 - 320 of 15118 results

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Yellow Cameleon-Nano15/pcDNA3

Plasmid

#51961

Purpose

mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano15

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
CMV-NanoLuc-BRAF-CAT

Plasmid

#194772

Purpose

Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)

Use

Tags

NanoLuc

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pmBanana-Hyg-CTCF-NtermNES-N1

Plasmid

#131850

Purpose

Expresses the [N-terminus of human CTCF (a.a. 2-265)]-mBanana with a nuclear export signal in mammalian cells

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Use

Tags

mBanana

Expression

Mammalian

Mutation

deleted amino acids 796-2181 (the N-terminus is l…

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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miRFP718nano-based inflammation reporter

Plasmid

#195753

Purpose

Reporter of inflamation

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

NF-kB-RE-miRFP718nano

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano140/pcDNA3

Plasmid

#51966

Purpose

mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano140

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MYO10-HMM-Nanotrap

Plasmid

#87255

Purpose

Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

GFP-nanotrap

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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nanobody(AntiGFP)-HaloTag-iLID

Plasmid

#191453

Purpose

Localizing iLID with GFP-tagged proteins

Depositor

Dorus Gadella

Article

Mahlandt et al bioRxiv 2022.11.24.517828

Insert

nanobody(AntiGFP)-HaloTag-iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano50/pcDNA3

Plasmid

#51964

Purpose

mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano50

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHDR_NANOS3-T2A-mVenus-PuroTK

Plasmid

#222903

Purpose

Homology directed repair template for knocking in mVenus reporter to NANOS3.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mVenus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP1)/pcDNA3

Plasmid

#51992

Purpose

mammalian expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
C1-82 pComb3xss anti-cFos nanobody

Plasmid

#145829

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pcDNA3

Plasmid

#67661

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Cyan Nano-lantern/pcDNA3

Plasmid

#67660

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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REV human NANOS3 TALEN

Plasmid

#59723

Purpose

TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only
C2-77 pComb3xss anti-cFos nanobody

Plasmid

#145837

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-77 (FOS L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-mitochondria/pcDNA3

Plasmid

#51973

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only
pTriEx-mCherry-LINXa3-nano

Plasmid

#73616

Purpose

Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3-nano

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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S1-71 pComb3xss anti-cFos nanobody

Plasmid

#145832

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S1-71 (FOS L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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