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Plasmid#103087PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB5JU35(Cas9 coding gene from gamma proteobacterium HTCC5015)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-B5XLC1
Plasmid#103088PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB5XLC1(Cas9 coding gene from Streptococcus pyogenes serotype M49 (strain NZ131))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-C4ZA16
Plasmid#103090PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertC4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PIAL2M-wt
Plasmid#67909PurposeInducible expression of PIAL2 in E.coliDepositorInsertPIAL2 (AT5G41580 Mustard Weed)
UseTagsMBPExpressionBacterialMutationE281-A496PromoterP-lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-pA-M.EcoGII
Plasmid#192873PurposepA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578DepositorInsertpA-M.EcoGII
UseTags3X Flag TagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pSems-leader-mEGFP-MBP-TMD(ALA7)
Plasmid#193051PurposeExpression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.DepositorInsertALA7-TMD
UseTagsIg k-chain leader sequence, MBP, and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer
Plasmid#176589PurposeEndogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Chesapeake Bay) locus
UseTagsExpressionBacterialMutationTruncated CRISPR array to two direct repeats, rep…PromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ISG15 C-term. Domain intein/chitin binding domain (79-156)
Plasmid#110760PurposeBacterial expression of ISG15 C-term.DepositorInsertISG15 (ISG15 Human)
UseTagsintein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer
Plasmid#176588PurposeEndogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Delaware Bay) locus
UseTagsExpressionBacterialMutationReplaced spacer in CRISPR array with Fn spacerPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C
Plasmid#114613PurposeBacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeatDepositorInsertHuntingtin Exon1 (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationA2C, A60C; 23 polyQ repeatPromoterAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Htt2-90-43Q-P90C
Plasmid#114626PurposeBacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeatDepositorInsertHuntingtin Exon1 fragment (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationP90C; 43 polyQ repeatPromoterAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Htt2-90-15Q-P90C
Plasmid#114611PurposeBacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeatDepositorInsertHuntingtin Exon1 fragment (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationP90C; 23 polyQ repeatPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)
Plasmid#110761PurposeBacterial expression of proISG15DepositorInsertISG15 (ISG15 Human)
UseTagsintein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q
Plasmid#114617PurposeBacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeatDepositorInsertHuntingtin Exon1 fragment (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationA2C; 23 polyQ repeatPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q-A2C
Plasmid#114622PurposeBacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeatDepositorInsertHuntingtin Exon1 (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationA2C; 43 polyQ repeatPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q-A2C-A60C
Plasmid#114623PurposeBacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 43 polyQ repeatDepositorInsertHuntingtin Exon1 (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationA2C, A60C; 43 polyQ repeatPromoterAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q
Plasmid#114627PurposeBacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 49 polyQ repeatDepositorInsertHuntingtin Exon1 fragment (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationA2C; 49 polyQ repeatPromoterAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Htt2-90-49Q-P90C
Plasmid#114631PurposeBacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeatDepositorInsertHuntingtin Exon1 fragment (HTT Human)
UseTagsHis6-Ssp DnaB inteinExpressionBacterialMutationP90C; 49 polyQ repeatPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only