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pMRXIP-GFP-2xFYVE

Plasmid

#221750

Purpose

Stable expression of GFP-2xFYVE in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

tandem HRS FYVE domain

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-Sik-YF

Plasmid

#210723

Purpose

Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Expression

Mammalian

Mutation

Tyrosine 447 changed to Phenylalanine

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pQE60 G121A wgMDH

Plasmid

#204252

Purpose

Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

G121A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R196E wgMDH

Plasmid

#204272

Purpose

Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196E

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P123A wgMDH

Plasmid

#204254

Purpose

Bacterial expression of P123A wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

P123A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R130Q wgMDH

Plasmid

#204268

Purpose

Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130Q

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 G127F wgMDH

Plasmid

#204264

Purpose

Bacterial expression of G127F wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

G127F

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P126R wgMDH

Plasmid

#204261

Purpose

Bacterial expression of P126R wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

P126R

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 G127A wgMDH

Plasmid

#204263

Purpose

Bacterial expression of G127A wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

G127A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 M66L wgMDH

Plasmid

#204247

Purpose

Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

M66L

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 G127P wgMDH

Plasmid

#204265

Purpose

Bacterial expression of G127P wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

G127P

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 G121F wgMDH

Plasmid

#204253

Purpose

Bacterial expression of G121F wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

G121F

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 M91Q wgMDH

Plasmid

#204250

Purpose

Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

M91Q

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 D87L wgMDH

Plasmid

#204248

Purpose

Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

D87L

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P126G wgMDH

Plasmid

#204260

Purpose

Bacterial expression of P126G wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

P126G

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P123G wgMDH

Plasmid

#204256

Purpose

Bacterial expression of P123G wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

P123G

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 P123F wgMDH

Plasmid

#204255

Purpose

Bacterial expression of P123F wgMDH with IPTG induction - Active site loop mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

P123F

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-hArfaptin1

Plasmid

#206402

Purpose

Mammalian expression of hArfaptin1

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin1 (ARFIP1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-hArfaptin2

Plasmid

#206403

Purpose

Mammalian expression of hArfaptin2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

Arfaptin2 (ARFIP2 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A D197,199N

Plasmid

#195704

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9 with D1…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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