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Showing: 4801 - 4830 of 8424 results

  1. pREP4xN_ccdB

    Plasmid
    #79022
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79022-plasmid-map-sequence-id-147779

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  2. pREP3xN_ccdB

    Plasmid
    #79021
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79021-plasmid-map-sequence-id-151965

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  3. pREP82xN_ccdB

    Plasmid
    #79026
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79026-plasmid-map-sequence-id-147783

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  4. pREP81xN_ccdB

    Plasmid
    #79025
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79025-plasmid-map-sequence-id-151959

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  5. pTU2-a_Sv5KA_amilCP

    Plasmid
    #204050
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var5 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    204050-plasmid-map-sequence-id-437162

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  6. pTU2-a_Sv3KA_amilCP

    Plasmid
    #204052
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var3 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204052-plasmid-map-sequence-id-418172

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  7. pTU2-a_Sv6KA_amilCP

    Plasmid
    #204049
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var6 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204049-plasmid-map-sequence-id-418169

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  8. pTU2-a_Sv7KA_amilCP

    Plasmid
    #204048
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var7 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204048-plasmid-map-sequence-id-418164

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  9. pTU2-a_Sv4KA_amilCP

    Plasmid
    #204051
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var4 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204051-plasmid-map-sequence-id-413880

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  10. pANY4

    Plasmid
    #164897
    Purpose
    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164897-plasmid-map-sequence-id-327986

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  11. SP65U1-135a-2

    Plasmid
    #105820
    Purpose
    plasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid
    Depositor
    Cecilia Mannironi
    Insert
    MIRNA135A2 (Mir135a-2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U1
    Available since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    105820-plasmid-map-sequence-id-202522

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  12. pREP41xN_ccdB

    Plasmid
    #79023
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79023-plasmid-map-sequence-id-151952

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  13. pREP42xN_ccdB

    Plasmid
    #79024
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79024-plasmid-map-sequence-id-147781

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  14. pWPI

    Plasmid
    #12254
    Purpose
    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.
    Depositor
    Didier Trono
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EMCV IRES-EGFP cassette
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12254-plasmid-map-sequence-id-429661

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  15. R6K_tac_mCherry_ChInt

    Plasmid
    #187382
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherry
    Depositor
    Roi Avraham
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    187382-plasmid-map-sequence-id-371124

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  16. R6K_tac_GFPmut3_ChInt

    Plasmid
    #187381
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3
    Depositor
    Roi Avraham
    Insert
    GFPmut3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187381-plasmid-map-sequence-id-370413

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  17. cEF1a-LSL-GFP

    Plasmid
    #135672
    Purpose
    Introduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135672-plasmid-map-sequence-id-325851

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  18. pAW8-aid-eDSR-Turg1:kanMX6

    Plasmid
    #169362
    Purpose
    C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169362-plasmid-map-sequence-id-333207

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  19. pLSU1/t35s[AtPsaK]:MCS+:NosT

    Plasmid
    #212159
    Purpose
    This binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (truncated Arabidopsis thaliana PsaK)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212159-plasmid-map-sequence-id-422849

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  20. pAW8-aid-spo5DSR-Turg1:kanMX6

    Plasmid
    #169358
    Purpose
    C-terminal aid-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169358-plasmid-map-sequence-id-333930

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  21. pANY8

    Plasmid
    #164900
    Purpose
    Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-ELP-Intein-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164900-plasmid-map-sequence-id-330483

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  22. pJM101: pHps1 RFP on pCM66T

    Plasmid
    #74740
    Purpose
    pCM66T with promoter pHps1 driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    pHps1 (extracted from Methylovorus glucosetrophus…
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74740-plasmid-map-sequence-id-146986

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  23. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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  24. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    58807-plasmid-map-sequence-id-91105

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  25. pBHt2G-RFP

    Plasmid
    #107162
    Purpose
    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Type
    Empty backbone
    Use
    Fungal grobacterium transformation plasmid, teste…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    107162-plasmid-map-sequence-id-205976

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  26. pRSFDuet-galK

    Plasmid
    #193727
    Purpose
    Contains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galK
    Depositor
    Martin Buck
    Insert
    galK (galK E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23113
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193727-plasmid-map-sequence-id-381922

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  27. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

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  28. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

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  29. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid
    #169355
    Purpose
    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169355-plasmid-map-sequence-id-333206

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  30. pAW8-aid-V5-eDSR-Turg1:kanMX6

    Plasmid
    #169365
    Purpose
    C-terminal aid-V5-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    1XV5
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169365-plasmid-map-sequence-id-333205

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Showing: 4801 - 4830 of 8424 results