We narrowed to 12,247 results for: ALP

1. pET41SIII-ATF6(1-373)

   Plasmid

   #171074

   Purpose

   Expresses of ATF6α(1-373) in bacteria

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   ATF6α (ATF6 Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav2.2 HA pCAGGS

   Plasmid

   #206052

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCellFree_G03 AKT1

   Plasmid

   #67102

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. Gal4pBS134-Pleum

   Plasmid

   #60339

   Purpose

   Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. TMED9-bio-His

   Plasmid

   #51855

   Purpose

   Expresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TMED9 (TMED9 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TMED4-bio-His

   Plasmid

   #51854

   Purpose

   Expresses full-length Transmembrane emp24 domain-containing protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TMED4 (TMED4 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mouse E-cadherin GFP (423)

   Plasmid

   #67937

   Purpose

   Mammalian expression of E-cadherin-GFP

   Depositor

   Alpha Yap

   Article

   Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD8-GFP-FKBP

   Plasmid

   #100751

   Purpose

   Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   CD8A (CD8A Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Truncated

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIL-eGFP-shACTN1

   Plasmid

   #52676

   Purpose

   shRNA against α-actinin-1 with eGFP transfection marker

   Depositor

   Johannes Hell

   Article

   Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

   Insert

   ACTN1 (Actn1 Rat)
   

   Use

   RNAi

   Tags

   CMV-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PIK3CA - Exon9 E545K

    Plasmid

    #16642

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E545K; see note below

    Promoter

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. FR_HNF4A2

    Plasmid

    #31100

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pECE HA AMPKa1 WT

    Plasmid

    #69504

    Purpose

    Expresses AMPK alpha1 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid

    #49333

    Purpose

    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    CMV

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Human a4 nicotinic subunit crystallization construct

    Plasmid

    #85841

    Purpose

    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

    Insert

    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ACTN3-pEGFP

    Plasmid

    #52670

    Purpose

    Overexpression of GFP-tagged α-actinin-3

    Depositor

    Johannes Hell

    Article

    Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

    Insert

    ACTN3 (ACTN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX462-hPRKAA1-gRNA_B

    Plasmid

    #74375

    Purpose

    gRNA_B to knockout human AMPK alpha 1 using Cas9n

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX462-hPRKAA1-gRNA_A

    Plasmid

    #74374

    Purpose

    gRNA_A to knockout human AMPK alpha 1 using Cas9n

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pM2AH + mSlo1

    Plasmid

    #16152

    Depositor

    Larry Salkoff

    Article

    Butler et al Science. 1993 Jul 9. 261(5118):221-4.

    Insert

    Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pECE HA AMPKa1 AS

    Plasmid

    #69831

    Purpose

    Expresses Expresses AMPK alpha 1 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSIL-eGFP-shACTN4

    Plasmid

    #52679

    Purpose

    shRNA against α-actinin-4 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN4 (Actn4 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge