We narrowed to 8,425 results for: TIL;

1. pET21-pelB-LaG-26

   Plasmid

   #172751

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-26
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDR111_Bgal NLS

   Plasmid

   #188396

   Purpose

   Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Tags

   PhoD secretion peptide and SV40 NLS

   Expression

   Bacterial

   Promoter

   Phyper spank

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C2-TetR-KRf-TetR

   Plasmid

   #216270

   Purpose

   Construct 3: TetR - Lipid binding domain KRφ - TetR

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   TetR - Lipid binding motif (KRf) - TetR
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAST-GFP-RpL10Ab

   Plasmid

   #71729

   Purpose

   Expression of Drosophila melanogaster Rpl10Ab-GFP fusion

   Depositor

   Herman Dierick

   Article

   Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

   Insert

   RpL10Ab (RpL10Ab Fly)
   

   Tags

   EGFP

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

5. IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

   Plasmid

   #121750

   Purpose

   pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   3x HA epitope tag + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC292

   Plasmid

   #118881

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
   

   Tags

   mRuby3, HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-kanMX6-PGAL1-GFP

   Plasmid

   #41614

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Available Since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-kanMX6-P3nmt1-3HA

   Plasmid

   #39283

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Mutation

   -1163 to +6 of nmt1 locus

   Available Since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. CVS-N2c-tdTomato-FlpO

   Plasmid

   #172375

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   tdTomato + FlpO
   

   Use

   Neurotropic virus

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIC292

    Plasmid

    #51405

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska2 IMAGE 4685972 (SKA2 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUb dRxCas13d + RxCas13d guide RNA

    Plasmid

    #176304

    Purpose

    Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    RxCas13d
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    R239A, H244A, R858A, H863A

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiEKAR2G1

    Plasmid

    #40177

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC291

    Plasmid

    #118867

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mRuby3

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-21

    Plasmid

    #172749

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-21
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaG-37

    Plasmid

    #172756

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-37
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. KX002: pMVP/PB-DEST

    Plasmid

    #121869

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223 PAK5

    Plasmid

    #60532

    Purpose

    Donor vector for human Pak5

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pSunS

    Plasmid

    #171672

    Purpose

    SunS expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMuLE ENTR H1-miR-30 L1-R5

    Plasmid

    #62181

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only