We narrowed to 11,264 results for: Vars

1. gamma-tubulin (1-335)

   Plasmid

   #226519

   Purpose

   Expressing a fragment of gamma-tubulin containing gamma-tubulin GTPase domain

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

   Insert

   TUBG1 N-terminal amino acids 1-335 (TUBG1 Human)
   

   Use

   Pcdna3.1

   Tags

   Expression

   Mammalian

   Mutation

   Deleted amino acids 334-451 and introduced the fo…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBT-RP2.2

   Plasmid

   #189842

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   GFP GM2 variant and mRFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBT-RP2.3

   Plasmid

   #189843

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   GFP GM2 variant and mRFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2CG NBT:NRGIIIa_R551Q:polyA

   Plasmid

   #193013

   Purpose

   Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

   Depositor

   William Talbot

   Article

   Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

   Inserts

   NBT
   NRG1 type IIIa R551Q (NRG1 Human)
   

   Use

   Transposon transgenesis

   Tags

   Expression

   Mammalian

   Mutation

   ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Donor_N_FH_Piwi

   Plasmid

   #186651

   Purpose

   Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9_ANKRD1_sgRNA2

   Plasmid

   #186669

   Purpose

   Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. S+Q111R+N122D

   Plasmid

   #167175

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+Q111R+N122D
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed Q at 111 to R and changed N at 122 to D

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. S+10subs

   Plasmid

   #167176

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+10R
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed A at 112 to T; E at 116 to D; I at 135 to…

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. S+12subs

   Plasmid

   #167177

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+12R
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed Q at 111 to R; A at 112 to T; E at 116to …

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM33_Nsp3e_NAB

    Plasmid

    #156473

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3e_NAB (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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