We narrowed to 4,489 results for: TIL;

1. KK802: pMVP (L5-L4) Zeo-P2A

   Plasmid

   #121719

   Purpose

   pMVP L5-L4 entry plasmid, contains Zeo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Zeo selection marker linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Zeo-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. IG901: pMVP (L5-L4) FLAG epitope tag

   Plasmid

   #121701

   Purpose

   pMVP L5-L4 entry plasmid, contains FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   FLAG epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. IH101: pMVP (L5-L4) OLLAS epitope tag

   Plasmid

   #121703

   Purpose

   pMVP L5-L4 entry plasmid, contains OLLAS epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   OLLAS epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. IX104: pMVP (L5-L4) myc epitope tag

   Plasmid

   #121704

   Purpose

   pMVP L5-L4 entry plasmid, contains myc epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   myc epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KG703: pMVP (L5-L4) 6x His epitope tag

   Plasmid

   #121706

   Purpose

   pMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   6x His epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. IO801: pMVP (L5-L4) 3x FLAG epitope tag

   Plasmid

   #121702

   Purpose

   pMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   3x FLAG epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVR2

   Plasmid

   #84719

   Purpose

   E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Expression

   Bacterial

   Mutation

   12 nt stretch lacking A's inserted before 5&…

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC176

   Plasmid

   #118891

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107321

   Purpose

   Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1333S) is fused to SaCas9

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. St1Cas9_LMD-9_sgRNA_pUC19

    Plasmid

    #110627

    Purpose

    U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Insert

    Empty St1Cas9 LMD-9 sgRNA backbone
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

    Plasmid

    #80325

    Purpose

    Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

    Depositor

    Daniel Schoenberg

    Article

    Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

    Insert

    PXDNL-003 (PXDNL Human)
    

    Tags

    Biotin

    Expression

    Mammalian

    Mutation

    H170A and H256A

    Promoter

    CMV

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. AP54-5

    Plasmid

    #65599

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APs12
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pMuLE ENTR SFFV tdTomato L5-L2

    Plasmid

    #62155

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    tdTomato
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMuLE ENTR CMV iRFP L3-L2

    Plasmid

    #62159

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    iRFP
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMuLE ENTR SV40 PuroR L5-L2

    Plasmid

    #62165

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Puromycin
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMuLE ENTR U6 n.s shRNA L1-L4

    Plasmid

    #62136

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    non-silencing (scrambled) shRNA
    

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMuLE ENTR U6 n.s shRNA R4-R3

    Plasmid

    #62137

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    non-silencing (scrambled) shRNA
    

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMuLE ENTR U6 n.s shRNA L5-L2

    Plasmid

    #62139

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    non-silencing (scrambled) shRNA
    

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE ENTR SV40 eGFP L3-L2

    Plasmid

    #62145

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    eGFP
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMuLE ENTR 7SK-miR-30 R4-R3

    Plasmid

    #62121

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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