We narrowed to 30,515 results for: Ide
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Plasmid#22793DepositorInsertMutant miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
SAM-DNMT3A-inactive+sgAAVS1_118
Plasmid#213171PurposeVector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.DepositorInsertsgAAVS1_118 (AAVS1 Human)
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterE1Fa and U6Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA
Plasmid#177360PurposeAAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoterDepositorInsertstdTomato
Chimeric guide for SaCas9
UseAAVTagsP2A and a tetracycline-controlled transactivator …ExpressionMammalianMutationPromoterSynapsine promoter and U6 promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA
Plasmid#177365PurposeAAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expressionDepositorInsertstdTomato
Chimeric guide for SaCas9
UseAAVTagsP2A and a tetracycline-controlled transactivator …ExpressionMammalianMutationPromoterCMV and U6 promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
B-HypaR-SpCas9
Plasmid#126764PurposeExpression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.DepositorInsertB-Hypa2SpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationR661A; N692A; M694A; Q695A; H698A; amino acids 10…PromoterCBhAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LANS1-Set2
Plasmid#122002PurposeFLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeastDepositorInserthistone methyltransferase SET2 (SET2 Budding Yeast)
UseSynthetic BiologyTagsFLAGExpressionYeastMutationSet2 point mutations K538G, K539S, R549G, K550S, …PromoterADH1Available sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p35S_GFP-EVDinter-GUS
Plasmid#167123PurposePlant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.DepositorInsertEVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
UseTagsGUS and mGFP5ExpressionPlantMutationPromoterCaMV 35SAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3e_NAB
Plasmid#156473PurposeBacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tagDepositorInsertNsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-TOM20MTS-DDRGK1-dTM-HA
Plasmid#139861PurposeLentiviral expression of TOM20MTS-DDRGK1-dTM-HADepositorInsertTOM20-MTS-DDRGK1-dTM (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationrecombinant fusion of mitochondria-targeting sign…PromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM304_YFP-IqgC_mRFP-Raf1(RBD)
Plasmid#128698Purposesimultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cellsDepositorInsertsiqgC (full length)
Raf1 kinase (aa 50-132)
UseDictyostelium discoideum expression vectorTagsYFP and mRFPExpressionMutationnonePromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL5 sgRNA(MS2)-MS2-hA3a D131A
Plasmid#112131Purposeempty sgRNA cloning vector with MS2-humanA3a D131ADepositorInserthuman Apobec3a D131A mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationD131A mutant in hA3aPromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-KRAB-Hygro donor
Plasmid#199621PurposeDonor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-idCas9-KRAB-Hygro
UseTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-ccdB-boxB-tBE-V5-mA3
Plasmid#171693PurposeExpresses tBE-V5-mA3 in mammalian cellsDepositorInsertccdB-sgRNA scaffold-boxB-tBE-V5-mA3
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and EF1aAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA StreptagII 3C NIS EABR
Plasmid#234988PurposeFor production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surfaceDepositorInsertNIS-EABR (SLC5A5 Human)
UseTagsHA, Streptag-II, HRV 3C siteExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs GFP-LC3-RFP
Plasmid#117413PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-PA50
Plasmid#84906Purposeinduce expression of dipeptide repeat PA in yeastDepositorInsertPA50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-GA50
Plasmid#84907Purposeinduce expression of dipeptide repeat GA in yeastDepositorInsertGA50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-21b(+)-Is-PETase-W185A
Plasmid#112204PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationW185APromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_P2A_Puro
Plasmid#211364PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only