We narrowed to 1,105 results for: Ide
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TypeCollection... top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top C. elegans ID Plasmid...Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Zebrafish ID Plasmid...Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Parasite ID Plasmid...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...
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CRISPR Plasmids - Mammalian Expression
TypeCollection... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI...diverse populations of mutants for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI... and are potentially heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection... top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top C. elegans ID Plasmid...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR... -
CRISPR Plasmids - Bacteria
TypeCollection...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse... A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting... -
AAV Molecular Tools
TypeCollection...tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118... purification (which can be used for viral TRAP). ID Name Expression System Activity Serotype PI 98747...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509... encode tools that can be used for cell ablation. ID Name Expression System Activity Serotype PI 124364...the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System Activity Serotype PI 61592...tools that can be used for cell or protein labeling. ID Name Expression System Activity Serotype PI 149415...These AAV encode various genes for overexpression. ID Name Expression System Activity Serotype PI 58376... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid... Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR... -
CRISPR Plasmids - Yeast
TypeCollection...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI... A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable Marker PI... -
Serotype Testing AAV
TypeCollection...material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 37825-AAV1.T 20 ...material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 50465-AAV1.T 20 ...please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample is denoted with...with a .T suffix (for example, AAV1.T). The catalog ID for the 100 µL sized sample does not have the suffix... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI... or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR... -
CRISPR Plasmids - Base Edit
TypeCollection...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid...Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Zebrafish ID Plasmid...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window...available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR... -
CRISPR Plasmids - Plants
TypeCollection...protein can be paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication... A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI... -
Institute for Protein Innovation
TypeCollection...toolset for scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity... epitope tags and other common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity...used to create or express plasmid-based antibodies. ID Plasmid Description...the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit, outside the ligand-binding...ligand-binding pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific subunit pairs,... -
CRISPR Plasmids - Drosophila
TypeCollection...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick ...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog... base-to-base conversions using a prime editing guide RNA (pegRNA). The pegRNA directs the nickase to ... -
Recombinases AAV Preps
TypeCollection... information about these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551...AAV.TBG.PI.Cre.rBGe TBG none 8 James M. Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV...-DreO-bGHpA Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV...none 8 Karl Deisseroth Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV...pAAV-EF1a-iFlpV EF1a none 1, PHPeB Hongkui Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert ...Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog... -
CRISPR Plasmids - Prime Edit
TypeCollection...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed...Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI...deletions, and point mutations using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR... -
Open Enzyme Collection
TypeCollection...RNA Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase... Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees...Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506...NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) ...Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase...RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert 165509 pOpen-TEV protease TEV...consists of more than 75 genes for enzymes that are considered "workhorses" of molecular biology, including ... -
Lentiviral Prep Service
TypeCollection.... For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection...DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity...transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422...VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114...sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637... -
Deisseroth INTRSECT Collection
TypeCollection... and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...pAAV-CaMKIIa-Flpo None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Cre Yes Dual recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...Cre Yes Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... -
Antibody Guide
TypeCollection... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...This method uses peptide identification by proteomics (Capture MS) to validate the identity of proteins recognized...first identify your assay type and what species your protein of interest comes from. Then consider the ...In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents...Antibodies can be used in the lab to quantify, identify, separate, and locate proteins of interest. The... between antibodies, allows for the antibody’s identity to be easily confirmed via sequencing, and enables...