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We narrowed to 17 results for: Ide

Showing: 1 - 17 of 17 results

  1. Science Guides

    Type
    Guide
    ...Collection of guides that describe how popular scientific techniques work... Science Guides Addgene has created... created the following guides to help you start learning about various scientific concepts related to ...to our plasmid collection. Find links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics...molecule, can be used to capture, quantify, and identify proteins of interest. Read More Chemogenetics ... sets. Read More Viral Vectors Find educational guides to learn more about the different types of viruses...

  2. Adenovirus Guide

    Type
    Guide
    ...Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production Integration Adenoviral...homologs, especially when having to express them alongside a guide RNA and other regulatory elements. The packaging...production, in trans refers to genetic elements provided outside of the plasmid containing the gene of interest... vector guide to learn about adenoviral vector components, production and common uses. Guides... Guides Adenoviral...a protein capsid and the viral genetic material inside. Adenoviral genomes range between 26–45 kb in length... terminal repeats (ITRs). Adenoviral genes are divided into early (E1A, E1B, E2, E3, E4) and late (L1,...

  3. Chemogenetics Guide

    Type
    Guide
    ... Science Guides Chemogenetics Guide Chemogenetics Guide Jump To: Chemogenetics Guide Early Chemogenetic...) is a widely-used tool for achieving in vivo expression of chemogenetic receptors, and a wide variety...and this must be considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors ...Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity. Science...the application of a small molecule ligand. The ideal chemogenetic tools are unresponsive to native ligands...signaling. GPCRs have been shown to be involved in a wide variety of biological processes, including initiating...of constitutive activity, making them less than ideal. DREADDs Designer Receptors Exclusively Activated...

  4. CRISPR Guide

    Type
    Guide
    ...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...making CRISPR perfect for genome-wide screens. This guide will provide a basic understanding of CRISPR ...genomic target of the gRNA can be any ~20 nucleotide sequence, provided it meets two conditions: The sequence...discuss more Cas9 fusion tools later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced...Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes...with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes...some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) ...

  5. Optogenetics Guide

    Type
    Guide
    ... Science Guides Optogenetics Guide Optogenetics Guide Jump To: Optogenetics Guide Introduction Microbial...have been created and identified in other species - by acting as light-gated chloride channels, these variants... mutations 540 Channelrhodopsins: chloride channels iChloC Chloride-conducting channel, CrChR2 with mutations...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...ions into or out of the cell. Scientists have identified a variety of naturally occurring microbial opsins...stimulated. Channelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool, is also a blue light ...been expanded by scientists through both the identification of novel ChRs from other algal species and ...

  6. Antibody Guide

    Type
    Guide
    ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...This method uses peptide identification by proteomics (Capture MS) to validate the identity of proteins recognized...first identify your assay type and what species your protein of interest comes from. Then consider the ...In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents...Antibodies can be used in the lab to quantify, identify, separate, and locate proteins of interest. The... between antibodies, allows for the antibody’s identity to be easily confirmed via sequencing, and enables...

  7. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral...production, in trans refers to genetic elements provided outside of the plasmid containing the gene of interest...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...mutations, or treatments into cells, and has been widely used in the research community for decades. Wild-type...in a host, and exhibit low immunogenicity. This guide contains many viral vector-specific terms and acronyms...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses ...contains packaging gene env (usually VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G...

  8. Guide to Using Pooled Libraries

    Type
    Guide
    ... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries...amplification protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse... Libraries Jump To: Pooled Library Guide Introduction Library Amplification Types of Screens Resources...powerful tool for forward genetic screening, or identifying previously unknown genes that contribute to a...replicate for that plasmid. Library screens can be divided into two types: positive screens and negative screens...septicum alpha-toxin , and resistance to to the nucleotide analog 6-thioguanine . Next-generation sequencing...Positive Screen In a positive screen, the goal is to identify those cells that survive post-selection. The selective...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... Guides Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral...production, in trans refers to genetic elements provided outside of the plasmid containing the gene of interest...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...mutations, or treatments into cells, and has been widely used in the research community for decades. Wildtype...expression in a host and low immunogenicity. This guide contains many viral vector-specific terms and acronyms...about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors The genome of gamma-retroviruses...contains packaging gene env ; usually VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G ...

  10. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production Integration AAV...watching our Optogenetics 101 video or by visiting Addgene's Optogenetics Guide . Browse optogenetics plasmids...production, in trans refers to genetic elements provided outside of the plasmid containing the gene of interest...viral vector guide to learn about AAV vector components, production and common uses. Guides... Guides AAV Vector ...a protein capsid and the viral genetic material inside. The AAV genome is ~4.7 kb long and consists of...viral vectors for gene therapy applications. This guide contains many viral vector-specific terms and acronyms...

  11. Molecular Biology Reference

    Type
    Guide
    ...helix. The order of these 4 nucleotides makes up the genetic code and provides the instructions to make ...Scientists often “sequence DNA” to identify the order of these four nucleotide bases in a particular DNA strand... protocols and videos for specific techniques and tips! Addgene Protocols Addgene Video Library...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing... the conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. These... transcribed from DNA. DNA is comprised of 4 nucleotides or bases, adenine, thymine, cytosine, and guanine...amino acids. Each amino acid is encoded for by 3 nucleotides termed a codon. As there are only 20 natural ...

  12. Plan Your Experiment

    Type
    Guide
    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...selection marker to identify and enrich positive cells Packaging and envelope plasmids provide the necessary...see our Lentivirus Guide ) Stable, tunable expression of Cas9 and/or gRNA in a wide variety of mammalian... desired edit, ideally less than 10 bp away. In this case, it is necessary to identify the exact location... increase specificity, you can also consider using a high-fidelity Cas enzyme. In addition to off-target...the genome like never before. This section will provide a general framework to get you started using CRISPR...apply to using CRISPR in other organisms. First, consider the genetic manipulation that is necessary to ...

  13. Cloning

    Type
    Guide
    ..... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson...Addgene's reference guide on molecular cloning techniques, including information on seven commonly used...is likely that for any given project there is an ideal approach. This may be due to speed, cost, availability...method for your research project . The following guide will highlight several of the most popular cloning...DNA that already has restriction sites on either side of it into any plasmid that has the same sites in...sequence, which can make it difficult at times to identify compatible restriction enzymes, that cut your ...polymerases synthesize DNA molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together...

  14. Educational Resources

    Type
    Guide
    ...Fluorescent Proteins 101 Science Career Guide Videos Watch our collection of video content , including protocols...eBooks, science guides, videos, and protocols Educational...Techniques Protocols Find written and video protocols for a wide range of applications in molecular biology... Educational Resources From eBooks to videos and blog posts... to protocols, our educational resources cover a wide range of topics in molecular biology, laboratory...Creating Bacterial Glycerol Stocks Restriction Digests Guides Browse our in-depth instructional content on subjects...

  15. Addgene's eBook Collection

    Type
    Guide
    ...Select Science Career Guide November 2018 | First Edition About Science Career Guide November 2018 |...your gRNA and how to use pooled libraries in genome-wide screens. Close About Overlay Select Fluorescent ...Vectors 101 July 2024 | Second Edition About This guide to viral vectors contains an introduction to each... | First Edition About The guide includes information on the many career options in science, advice ...advice on mentoring and management, resources for identifying your transferable skills, and tips on the job...

  16. Promoters

    Type
    Guide
    ... are located within promoter regions, and they provide a stable binding site for RNA polymerase and transcription...Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal Promoter Further upstream from...prokaryotic promoters. Eukaryotic promoters span a wide range of DNA sequences. It is not unusual to have...

  17. Sequencing Primers

    Type
    Guide
    ...Primers? The primer sequences listed on the left are provided for your reference. Addgene does not distribute...primers based on the plasmid backbone and insert. To identify primers that may be useful in your sequencing ...
Showing: 1 - 17 of 17 results