We narrowed to 13,705 results for: gan

1. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

   Plasmid

   #47550

   Purpose

   Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   ttTi5605 sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLGCV

   Plasmid

   #49862

   Purpose

   Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).

   Depositor

   Jonathan Pettitt, Anne Glover

   Article

   Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118

   Insert

   firefly luciferase luc+ fused to GFP (S65C)
   

   Tags

   GFP (S65C)

   Expression

   Worm

   Promoter

   sur-5

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-gRNA(anti-cGAS)-Cas9-Puro

   Plasmid

   #130908

   Purpose

   gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-Cre sgAmotl2 v3

   Plasmid

   #158602

   Purpose

   lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Amotl2 sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pJH4203

   Plasmid

   #200160

   Purpose

   Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   twk-40 (twk-40 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Prig-3

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Diacylglycerol Kinase Zeta

   Plasmid

   #85454

   Purpose

   expression of Diacylglycerol Kinase Zeta

   Depositor

   Stephen Gee

   Article

   Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

   Insert

   Diacylglycerol Kinase Zeta (DGKZ Human)
   

   Tags

   3x HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS145

   Plasmid

   #179159

   Purpose

   Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

   Depositor

   Iva Greenwald

   Article

   Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Insert

   rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
   

   Expression

   Worm

   Mutation

   Flexon replacing 2nd intron of 2xNLS::GFP

   Promoter

   rps-27p

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiviral shRNA GEF-H1

   Plasmid

   #21477

   Depositor

   Richard Huganir

   Article

   Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

   Insert

   rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

9. Flag-V0D1

   Plasmid

   #210348

   Purpose

   Expresses ATP6V0D1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   V-ATPase V0D1 (ATP6V0D1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. NPY-mApple

    Plasmid

    #83498

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-2XFLAG,2XHA-CyclinF-puro

    Plasmid

    #32976

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

    Insert

    CYCLIN F (CCNF Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pMSCV-FKBP-RFP(pLxIS-PLPLR)

    Plasmid

    #221284

    Purpose

    Expression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    RFP (pLxIS-PLPLR) (Mkrn1 Synthetic)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-FKBP-TRIF(Nterm)

    Plasmid

    #221244

    Purpose

    Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    TRIF N-terminus (1-222) (Ticam1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. IRAK1mut-UTR

    Plasmid

    #15096

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    IRAK1 3'UTR (IRAK1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    4-bp substitutions in the miR-146 target sites

    Available Since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

    Plasmid

    #130275

    Purpose

    Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    Arch(D95N)
    

    Tags

    2x Myc-tag

    Expression

    Worm

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-IRES-hCD2-CD14

    Plasmid

    #133061

    Purpose

    Retroviral expression of mouse CD14

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    CD14 (Cd14 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GLG1-2Strep

    Plasmid

    #210362

    Purpose

    Expresses GLG1 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    GLG1 (GLG1 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBMN YFP-ltDHFR(DD)

    Plasmid

    #47080

    Purpose

    ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans

    Depositor

    Thomas Wandless

    Article

    Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:

    Insert

    YFP-ltDHFR(DD)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Mutation

    amino acids mutations: R12H, N37D, N59D, G67S, D1…

    Promoter

    LTR

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATG9A-2STrep

    Plasmid

    #210359

    Purpose

    Expresses ATG9A fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-LGC-53

    Plasmid

    #86399

    Purpose

    Mammalian expression of codon-optimized dopamine receptor LGC-53

    Depositor

    Steven Mennerick

    Article

    Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

    Insert

    LGC-53 (lgc-53 Nematode, Synthetic)
    

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression; Signal …

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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