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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 3241 - 3260 of 18824 results

  4. JPF0515

    Plasmid
    #124006
    Purpose
    Encodes pCMV-C driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-C-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124006-plasmid-map-sequence-id-252389

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  5. JPF0516

    Plasmid
    #124007
    Purpose
    Encodes pCMV-D driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-D-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124007-plasmid-map-sequence-id-252391

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  6. JPF0517

    Plasmid
    #124008
    Purpose
    Encodes pCMV-E driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-E-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124008-plasmid-map-sequence-id-252393

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  7. JPF0518

    Plasmid
    #124009
    Purpose
    Encodes pCMV-F driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-F-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124009-plasmid-map-sequence-id-252413

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  8. JPF0519

    Plasmid
    #124010
    Purpose
    Encodes pCMV-G driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-G-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124010-plasmid-map-sequence-id-252415

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  10. 3xFN-Tel-TAL/pMXs-neo

    Plasmid
    #63591
    Purpose
    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance gene.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038
    Insert
    3xFVN-Tel-TAL
    Use
    Retroviral and TALEN
    Tags
    3xFLAG-tag, V5-tag, and nuclear localization sign…
    Expression
    Mammalian
    Promoter
    LTR
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63591-plasmid-map-sequence-id-108371

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  13. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Publication
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  14. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid
    #114086
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114086-plasmid-map-sequence-id-231034

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  17. 5aa SunTag VP64 EVD

    Plasmid
    #115483
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    115483-plasmid-map-sequence-id-238821

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  19. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid
    #69233
    Purpose
    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Arginine 1335 is changed to Lysine
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    69233-plasmid-map-sequence-id-122312

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Showing: 3241 - 3260 of 18824 results