We narrowed to 9,561 results for: nls

1. nCas9-UGI control for Target-AID (pRZ1047)

   Plasmid

   #131299

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBUN411

   Plasmid

   #50581

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Mutation

   maize codon optimized

   Promoter

   OsU3p and Ubi1p

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHSN401

   Plasmid

   #50588

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Mutation

   maize codon optimized

   Promoter

   2×35Sp and AtU6-26p

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXR001: EF1a-CasRx-2A-EGFP

   Plasmid

   #109049

   Purpose

   CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Insert

   CasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   2A-eGFP, HA, and NLS

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBUN411

   Plasmid

   #50581

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Mutation

   maize codon optimized

   Promoter

   OsU3p and Ubi1p

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHSN401

   Plasmid

   #50588

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Mutation

   maize codon optimized

   Promoter

   2×35Sp and AtU6-26p

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpyCas9 PE2*

   Plasmid

   #169850

   Purpose

   Mammalian Expression, SpyCas9 prime editor, optimized NLS

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   spCas9 PE*
   

   Tags

   bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PRPF4.001.522.K45A.pc4

   Plasmid

   #137648

   Purpose

   Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.

   Depositor

   Tara Davis

   Insert

   PRPF4 (PRPF4 Human)
   

   Tags

   NLS and myc-(His)6

   Expression

   Mammalian

   Mutation

   001-522-K45A-pc4

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PRPF4.001.522.Y32A.pc4

   Plasmid

   #137646

   Purpose

   Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.

   Depositor

   Tara Davis

   Insert

   PRPF4 (PRPF4 Human)
   

   Tags

   NLS and myc-(His)6

   Expression

   Mammalian

   Mutation

   001-522-Y32A-pc4

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ-Cas12j2

    Plasmid

    #173924

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Insert

    NLS-Cas12j2-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCSN075

    Plasmid

    #166732

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCSN074

    Plasmid

    #166731

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PRPF4.001.522.K45A.pc4

    Plasmid

    #137648

    Purpose

    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.

    Depositor

    Tara Davis

    Insert

    PRPF4 (PRPF4 Human)
    

    Tags

    NLS and myc-(His)6

    Expression

    Mammalian

    Mutation

    001-522-K45A-pc4

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PRPF4.001.522.Y32A.pc4

    Plasmid

    #137646

    Purpose

    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.

    Depositor

    Tara Davis

    Insert

    PRPF4 (PRPF4 Human)
    

    Tags

    NLS and myc-(His)6

    Expression

    Mammalian

    Mutation

    001-522-Y32A-pc4

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHEE401E

    Plasmid

    #71287

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHEE401

    Plasmid

    #71286

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHEE2E-TRI

    Plasmid

    #71288

    Purpose

    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMP597

    Plasmid

    #134999

    Purpose

    Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-dLbCpf1-BE-YE1

    Plasmid

    #154145

    Purpose

    Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

    Insert

    dLbCpf1-BE-YE1 (dCas12a-BE-YE1)
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

    Promoter

    CMV

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ-NF-L1-dMb2Cas12a

    Plasmid

    #177683

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible foki-dmb2cas12a entry …

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge