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pMKV059

Plasmid

#133314

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPT

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+, IPT

Expression

Plant

Promoter

AtUbi10, 35S

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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lentiGuide-tagBFP

Plasmid

#217007

Purpose

lentiviral plasmid for gRNA cloning with selectable tagBFP expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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GalToxBFP

Plasmid

#68073

Purpose

mammalian expression of GC localized oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

GalT signal anchor transmembrane domain

Tags

moxBFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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MCP-M-MLV-RT

Plasmid

#181799

Purpose

MCP-M-MLV-RT (MS2-N55K-MMLVRT)

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MCP-M-MLV-RT

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSA351_SCP1_intron_eGFP

Plasmid

#206906

Purpose

Lentiviral eGFP reporter vector with SCP1 promoter.

Depositor

Stein Aerts

Article

Taskiran et al Nature. 2023 Dec 12. doi: 10.1038/s41586

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

SCP1

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-mCherry-CW3SL

Plasmid

#223662

Purpose

Expresses mCherry in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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JD630

Plasmid

#160394

Purpose

Vitis GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

Vitis GRF4-GIF1 chimera

Use

Entry clon

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMKV058

Plasmid

#133313

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+. WUS2

Expression

Plant

Promoter

AtUbi10, Nos

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pReg-RamR

Plasmid

#190610

Purpose

Expresses the RamR transcription factor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

RamR

Expression

Bacterial

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Pramr-GFP

Plasmid

#194148

Purpose

Contains a RamR-regulated promoter expressing GFP

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GFP

Expression

Bacterial

Promoter

Pramr

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Kpn1

Plasmid

#203297

Purpose

For expression of sgRNAs in a PlayBack Vector with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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JD689

Plasmid

#160396

Purpose

citrus GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

citrus GRF4-GIF1 chimera

Use

Entry clon

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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U6-petRNA

Plasmid

#181802

Purpose

petRNA cloning plasmid

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

petRNA-Kan

Use

CRISPR

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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Cofilin-pmCherryC1

Plasmid

#27687

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Cofilin (Cfl1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pSLQ11438

Plasmid

#183204

Purpose

pHR-EF1a-ABE8e-hyperdLbCas12a-NLS-mCherry-P2A-Puro-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

hyperdCas12 base editor

Use

CRISPR and Lentiviral

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IN

Plasmid

#60612

Purpose

Backbone for tet-inducible genes

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-NLS-M-MLV-RT

Plasmid

#181804

Purpose

pET28a-NLS-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

NLS-M-MLV-RT

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSL4164 (pEffector_IscB,ωRNA)

Plasmid

#210874

Purpose

Expresses GstIscB and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstIscB

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pReg-THP4

Plasmid

#190671

Purpose

Expresses an evolved tetrahydropapaverine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

THP4

Use

Synthetic Biology

Promoter

J23103

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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