We narrowed to 14,746 results for: TIM

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Cry2(1-531)-mCh-BAXS184E

Plasmid

#117238

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531-mCh-BAXS184E

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ173

Plasmid

#98410

Purpose

Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pPaxillin-Electra2

Plasmid

#184926

Purpose

Expresses paxillin fused to blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

paxillin-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-hNKD2

Plasmid

#174328

Purpose

encodes human NKD2 cDNA with a C-terminal GFP-fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

GFP tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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PE_CO

Plasmid

#190335

Purpose

Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE2 with codon-optimized reverse transcriptase

Expression

Mammalian

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mNG-sfTq2ox

Plasmid

#124219

Purpose

Cytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2ox

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-sfTq2ox

Expression

Mammalian

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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HBT_pcoCASphi_version1

Plasmid

#197931

Purpose

Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

FLAG tag and SV40 NLS and nucleoplasmin NLS

Expression

Plant

Promoter

35SPPDK promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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LAMP-CeNL_pcDNA3

Plasmid

#89514

Purpose

Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJH1371

Plasmid

#199631

Purpose

Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GPI

Tags

YFP

Expression

Worm

Promoter

Pregf-1

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAJM.969

Plasmid

#108537

Purpose

MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

MphRAM + EryR + PMph-YFP

Expression

Bacterial

Mutation

MphR: N94D

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pKB083

Plasmid

#181952

Purpose

IPTG-inducible expression of PhoQ

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)

Expression

Bacterial

Promoter

PhoQ-Ptac; lacI-PlacIq

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa

Plasmid

#78182

Purpose

Expresses ffDronpa E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

ffDronpa

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express2

Plasmid

#103999

Purpose

Plasmid encoding DsRed-Express2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CaMKIIbeta(A303R)

Plasmid

#21224

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CaMKIIbeta(A303R) (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

CaMKIIbeta(A303R) (Lab plasmid CF 0002)

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_NES_STf

Plasmid

#182001

Purpose

CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

NES-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pKeratin-Electra1

Plasmid

#179480

Purpose

Keratin labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Keratin-Electra1

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-AsCas12a-2AeGFP

Plasmid

#123638

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

AsCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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