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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 935 results for: TIM

Showing: 1 - 20 of 935 results

  1. KLF Research Plasmids

    Type
    Collection
    ...Rossi Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka...

  2. Bacterial Expression Systems

    Type
    Collection
    ... Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose Escherichia ...di-GMP Fluorescence (GFPmut2) Pseudomonas aeruginosa Tim Tolker-Nielsen 90217 pMV762-Peredox-mCherry NADH:...Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein Interactions Förster resonance...levels or to turn on expression only at a specific time. This collection contains plasmids whose expression...+ LacI Lactose/IPTG Corinebacterium glutamicum Timothy Lu 17972 pSE100 Pmyc1/TetO Anhydrotetracycline ...Prp PprpD2 Propionate Corinebacterium glutamicum Timothy Lu 36252 pRibo EcoHind Riboswitch Teophylline Escherichia...detect the presence of that small molecule and sometimes even quantify it. Bacterial one-hybrid (B1H) systems...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor for...or Cre-dependent) Cell-specific restoration of stimulus preference after monocular deprivation in the ...Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Nat Commun...Calcium GCaMP5 genetically encoded calcium sensor Optimization of a GCaMP calcium indicator for neural activity...variants (Constitutive or Cre-dependent) jYCaMP: an optimized calcium indicator for two-photon imaging at fiber... Indicator with an Expanded Dynamic Range by Optimization of Amino Acid Linkers between a Fluorescent ...Robust and Sensitive GFP-Based cGMP Sensor for Real Time Imaging in Intact Caenorhabditis elegans. Genetics...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MGC20661 CSF1 colony stimulating factor 1 (macrophage) MCSF, MGC31930 CSF1R colony stimulating factor 1 receptor...CSF3 colony stimulating factor 3 (granulocyte) G-CSF, GCSF, MGC45931 CSF3R colony stimulating factor 3 receptor...LXA4R FSHB follicle stimulating hormone, beta polypeptide - FSHR follicle stimulating hormone receptor FSHRO...MGC141920 TSHB thyroid stimulating hormone, beta CHNG4, TSH-BETA TSHR thyroid stimulating hormone receptor ...physical barriers such as the skin and mucosa, antimicrobial peptides and proteins in the blood such as interferons...presenting its cognate antigen along with co-stimulation activates the T cell, whereas T cell tolerance...are induced when the TCR is engaged without co-stimulation or with a low affinity antigen. TGFbeta In the...

  5. Antibody Guide

    Type
    Collection
    ...conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block some amines needed for conjugation...In those cases, thimerosal can be used as an antimicrobial agent. Other chemical interferences with conjugating...measurable light or color in response to a specific stimulus, can be conjugated to antibodies in order to allow...produces a colored product that accumulates over time which is relative to the amount of enzyme present...purchased already conjugated, which can save significant time in the lab. However, if the antibody you want is...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). This allows...protein or protein interactions in a specific timeframe or context. Immunoprecipitation (IP) IP uses immobilized...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Vary these times depending on the doubling time of the cell line used. NOTE: This incubation time allows ... such as cationic liposome-based transfection. Optimize transfection conditions for each cell line with...culture media after electroporation. Minimize the time between electroporation and transferring the solution...dilution at 30 cells per 96-well round-bottom plate. Optimize plating the cell type used at limiting dilution...1 min, 72 °C for 1 min), and 72 °C for 10 min. Optimize PCR conditions for each primer pair designed based...non-deletion” PCR primer pairs in a single reaction. Optimize multiplexing in a polyclonal population ( i.e.... the same PCR primers and reaction conditions optimized on the bulk cells (see step 6 ). Select the clones...

  7. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...turnaround time. Any timing expectations communicated by email will supersede the general estimated turnaround...any adjustments to the original order. Turnaround Time Production of a Packaged on Request material is ...verification, may not yet have been completed at the time of shipment. If any discrepancies are identified... we do not expect to meet the general turnaround time, Addgene will notify you by email before, or within...turnaround time above. After starting viral production, if we determine that we will not meet the turnaround...turnaround time we described here or by email, Addgene will contact you to offer some options for moving forward...

  8. Plan Your Experiment

    Type
    Collection
    ...be low, but generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere... and base editor variants often have different optimal editing windows. Be sure to refer to the literature...Using validated gRNAs can save your lab valuable time and resources when carrying out CRISPR experiments...gRNAs Once your target sequences are selected, it’s time to design your gRNA oligos and clone them into your...and express these components (Figure 2), and the optimal system will likely depend on your cell type. Figure...will work for your experiment) and the expression time (transient or stable). For plasmid and viral-based...best for your experiment. It may be necessary to optimize your delivery conditions, especially if you are...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA. The...contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons...Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ordered via the links below... S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single...contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...contains a single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as ...contains a single cassette expressing human codon-optimized SpCas9, driven by the truncated MeCP2 promoter...

  10. CRISPR Guide

    Type
    Collection
    ... an optimized gRNA for your application (see: Plan Your Experiment ). In addition to an optimized gRNA... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than...integrase landing site ( attB ) and the other to stimulate DNA repair. This can result in more efficient .... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...activators and targeting promoter regions. You might sometimes see these referred to together as CRISPRi/a. dCas9...

  11. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...integration efficiency but research groups continue to optimize these tools. The best characterized SSR systems...Expression By expressing the enzyme at specific times or locations using cell-specific promoters or inducible...inducible systems, you can precisely control and optimize expression of your gene of interest. Inducible...recombination of the DNA between target sites. Codon optimization: Modifying the DNA sequence of the recombinase...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. shRNA Expression... Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101...

  12. Brain Initiative Collection

    Type
    Collection
    ...application of innovative tools enabling large-scale real-time interrogation and monitoring of brain cells and ...198513-AAV1 pAAV_hSyn-PdCO-EGFP-WPRE Expresses optimized PdCO in frame with EGFP under control of human...198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE Expresses optimized PdCO in frame with EGFP under control of human..._EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of eukaryotic...soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase is often referred...luciferase is secreted and more stable, enabling time course experiments, but is too dim for some applications...modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression...luciferase and a Halo tag to assay mRNA turnover in real time. Nonsense-mediated decay reporter from Baird et ...® protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory...

  14. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Save time by ordering cloning grade DNA for over 200 of our most popular plasmids!...arrival of their plasmid from Addgene – reducing the time to experiments by removing the amplification and...order more than 2 µg of cloning grade DNA? At this time, to order more than 2 µg of a single plasmid, multiple...addgene.org . Don’t See What You’re Looking For? At this time, Addgene has made a limited number of plasmids available...

  15. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ... This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids...fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based ...Plasmids for constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid...Link opens in a new window) Sheff MA, Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces...

  16. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Inhibition Human Adamson 3rd ∼3 366 or 1,573 Activity-optimized genome-wide library Discontinued Knockout Human...Human Sabatini and Lander 3rd 10 178,896 Activity-optimized genome-wide library 1000000100 Knockout Human ...Knockout Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout ...Turner 3rd 10 8,260 Two plasmid human activity-optimized genome-wide library 1000000095 Knockout Human ...Lander 3rd 10 187,536 Two plasmid mouse activity-optimized genome-wide library 1000000096 Knockout Mouse ...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...repurposing endogenous systems that respond to natural stimuli like hormones or metal ions, scientists have adapted...promoter, and other tet- or dox-dependent promoters (sometimes generally called Ptet) have been designed by combining...Resnitzky et al., 1994). TRE3G promoter : also optimized into P TRE3GV for viral expression and P TRE3GS...in mammalian cells or other species by codon optimization of their sequence and eliminating interactions...Klaver, B., Berkhout, B., & Das, A. T. (2006). Optimization of the Tet-On system for regulated gene expression...

  18. Promega Plasmid Collection

    Type
    Collection
    ...high sensitivity. These assays are ideal for real-time monitoring of signaling pathways, investigating ... your specific purpose. HaloTag technology is optimized for various protein expression systems including...substrate. NanoBiT PPI assays enable kinetic, real-time analysis of protein interaction, association, and...you to detect PPI induction and inhibition in real time using full-length proteins or fragments. NanoBRET...

  19. AAV Packaged on Request

    Type
    Collection
    ...serves the needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities...approval of the MTA, and shipping, mean that the actual time from request to delivery could be up to 10 weeks...Production 6–8 weeks Quality is our priority. During this time, we amplify DNA, produce the prep, and perform comprehensive...information as soon as your order ships. Shipping time will depend on your location. Share Your Results...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ...embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, and transparency...proteins to visualize and track clones of cells over time. Examples of useful tools for studying cells that...clonal origin in zebrafish can be found below: Optimized QF-binary expression system for use in zebrafish...vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live...
Showing: 1 - 20 of 935 results