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rDNA-adj gRNA #1

Plasmid

#247548

Purpose

negative control for Crispri KD

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

negative control sgRNA targeting region adjacent to 5' end of ribosomal DNA repeats of distal junction

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX4T-2 GST-mouse Fis1 (ΔTM domain)

Plasmid

#252268

Purpose

Expression of GST-mouse Fis1 (ΔTM domain) in bacteria

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (deltaTM domain) (Fis1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

moue Fis1 1-121 amino acids

Promoter

tac

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (L110A)

Plasmid

#252265

Purpose

Retroviral expression of EGFP-mouse Fis1 (L110A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (L110A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating leucine 110 to alanine (L110A) in Fis1 r…

Promoter

CMV

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (W40A)

Plasmid

#252263

Purpose

Retroviral expression of EGFP-mouse Fis1 (W40A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (W40A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating tryptophan 40 to alanine (W40A) in Fis1 …

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pQCXIP EGFP-mouse Fis1 (V79A)

Plasmid

#252264

Purpose

Retroviral expression of EGFP-mouse Fis1 (V79A)

Depositor

David Chan

Article

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

mouse Fis1 (V79A) (Fis1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutating valine 79 to alanine (V79A) in Fis1 resu…

Promoter

CMV

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3159-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank4_COLO320 DM_ AT_sg-297-303])

Plasmid

#251116

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3157-{PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank2_COLO320 DM_ AT_sg-283-289])

Plasmid

#251114

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2977-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank1_PC3 DM_AT_sg-213-219])

Plasmid

#251109

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2978-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank2_PC3 DM_AT_sg-234-240])

Plasmid

#251110

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2982-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank7_PC3 DM_AT_sg-241-247])

Plasmid

#251112

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pSmBit-CHIP-G132N

Plasmid

#236065

Purpose

SmBit-CHIP expression with G132N substitution

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv [Preprint]. 2025 Feb 17:2025.02.

Insert

SmBit-CHIP-G132N (STUB1 Human)

Use

Luciferase

Tags

SmBit

Mutation

G132N

Promoter

HSV-TK

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBit-CHIP-K30A

Plasmid

#236066

Purpose

SmBit-CHIP expression with K30A substitution

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv [Preprint]. 2025 Feb 17:2025.02.

Insert

SmBit-CHIP-K30A (STUB1 Human)

Use

Luciferase

Tags

SmBit

Mutation

K30A

Promoter

HSV-TK

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C14A)-flag-tev-halo-his

Plasmid

#227159

Purpose

For T-REX experiments of CYP2A6 C14A mutant. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-halo-his

Plasmid

#227160

Purpose

For T-REX experiments of CYP2A6 C82A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C439A)-flag-tev-his

Plasmid

#227167

Purpose

For overexpression of CYP2A6 C439A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C295A)-flag-tev-halo-his

Plasmid

#227156

Purpose

For T-REX experiments of cyp-33e1 C295A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C439A)-flag-tev-halo-his

Plasmid

#227157

Purpose

For T-REX experiments of cyp-33e1 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Gab2-TurboID-MSCV-IRES-GFP

Plasmid

#240131

Purpose

Expresses Gab2-TurboID fusion in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

Gab2 (Gab2 Mouse)

Use

Retroviral

Tags

IRES-GFP and TurboID

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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TurboID-Gab2-MSCV-IRES-GFP

Plasmid

#240132

Purpose

Expresses TurboID-Gab2 fusion in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

Gab2 (Gab2 Mouse)

Use

Retroviral

Tags

IRES-GFP and TurboID

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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TurboID-NPM1-MSCV-IRES-GFP

Plasmid

#240133

Purpose

Expresses TurboID-NPM1 fusion in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

NPM1 (NPM1 Human)

Use

Retroviral

Tags

IRES-GFP and TurboID

Expression

Mammalian

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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