...kilobases. Nature Methods , 6(5), 343–345. https://doi.org/10.1038/nmeth.1318 (Link opens in a new window)...genome. Nature Methods , 7(11), 901–903. https://doi.org/10.1038/nmeth.1515 (Link opens in a new window)... Gibson Isothermal Assembly. bioRxiv . https://doi.org/10.1101/2020.06.14.150979 (Link opens in a new ...

... This will clear up any confusion later if your forget your bottle in the autoclave. Place the gel mix... you are working with any weird and wonderful organisms. While your samples are sterilizing in the autoclave...s possible that: The antibiotic broke down. You forgot to add the antibiotic. You added the antibiotic...

... accuracy of your plasmid, please email help@addgene.org and include: The sequencing result The original...mutation is not an artifact, please email help@addgene.org with the sequence, trace file, and primer used...

...RNAse-free tube. The mixture separates into a bottom organic layer, an interphase layer, and a top, aqueous ...

...pLKO.1 containing the 1.9kb stuffer, visit www.addgene.org/10878/ . Description Vector Element U6 Human...resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium (TRC): www.broad.mit.edu...

...within a laboratory setting is important for organization, and it is recommended that you keep a standard...

... page . Please feel free to email us at help@addgene.org with any questions. Protocols Video protocol ...

...own work. Please feel free to email us at help@addgene.org with any questions. Intro to the Lab Bench Introductory...

...is for labs that work with pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae...

...for virus purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly...

... precipitated protein particles Bottom phase - Organic phase (protein) Pipet the aqueous DNA layer and...

...duplicate Load 5 μL of each sample in duplicate. Do not forget to include a no template control ( NTC = master...

...standard curve or dilute the unknown sample (don’t forget to factor in that dilution when calculating the...

...DNA: guide RNAs targeting different genes in an organism's genome, for example. Amplification is usually...

...additional 10-fold dilution of the virus stock! Don’t forget to factor this into your calculations later (see...

...Lukas Morgan Model organisms are vital tools used by researchers around the globe. These organisms share...forefront by Thomas Hunt Morgan, who is known as the “father” of Drosophila research. Morgan discovered that genes...one of the simplest eukaryotic organism, is a commonly used model organism in scientific research. Yes, ...Looking for more model organisms? Check out Part two in the popular model organisms series, and read about...this blog post, we’ll cover five popular model organisms, but there are many more out there. The house... start with the most preferred mammalian model organism of many researchers: the mouse (Mus musculus)....Mice have many advantages as a mammalian model organism for scientists as they have a relatively short...

...covered five model organisms in a previous blog post, but here are five more model organisms that have left...emerging model organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia...as a model organism for over 60 years with a status as the most studied prokaryotic organism. With its ...https://doi.org/10.7554/elife.05826 Blum, M., & Ott, T. (2018). Xenopus: An Undervalued Model Organism to ...Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org/10.1159/000490898 Gibbs, R., ...amphibian model organisms. Dong wu xue yan jiu = Zoological research, 37(4), 237–245. https://doi.org/10.13918...https://doi.org/10.1038/s41467-018-04899-x Yokoyama, Maho. (2020, May 28). E. coli as a Model Organism. News-Medical...

...responsibilities Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst...great organization to develop these leadership skills in particular because every year it organized a “Committee...written a 5-part blog on the topic:  http://blog.addgene.org/management-for-scientists-managing-vs-leading... achieved by volunteering for a professional organization or a scientific society in your field. My leadership... in the long run was most beneficial for the organization as a whole. I came to understand that not only...only was I growing as a leader but the organization was growing too and with that would come necessary... had to be professional as I represented the organization, not just myself as a scientist. I learnt to...

...body is made up of organs, cells comprise compartments and structures, called organelles. Take a sneak peak...fluorescent microscopy - even in living cells and whole organisms. You can take advantage of the many Fluorescent... proteins” which are known to be a part of an organelle or involved in intracellular transport pathways...pathways, it is possible to map the internal organization of a cell. This mapping can be done by using antibodies...for commonly used markers for major mammalian organelles and transport pathways. It is best, though not...that traffics the fluorescent protein to your organelle of interest. Click on the gene names in the figure...Cell: A Molecular Approach. 2nd Edition. 3.  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn...

...populations of transgenic laboratory organisms but not wild organisms. For example, an sgRNA-only drive ...confinement seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety... Medical School. Scientists making transgenic organisms with Cas9 should be aware of the potential hazards... can spread even when costly. If even a single organism carrying a synthetic gene drive were to escape... extreme caution. Why does making transgenic organisms with Cas9 risk creating a gene drive? The simplest...idea or wanted to build a gene drive in a model organism would find our work and consequently use these...experiments in a geographic area where escaped organisms won't be able to find mates2. For example, ongoing...