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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15 results for: org

Showing: 1 - 15 of 15 results

  1. Gibson Assembly Protocol

    Type
    Protocol
    ...kilobases. Nature Methods , 6(5), 343–345. https://doi.org/10.1038/nmeth.1318 (Link opens in a new window)...genome. Nature Methods , 7(11), 901–903. https://doi.org/10.1038/nmeth.1515 (Link opens in a new window)... Gibson Isothermal Assembly. bioRxiv . https://doi.org/10.1101/2020.06.14.150979 (Link opens in a new ...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ... This will clear up any confusion later if your forget your bottle in the autoclave. Place the gel mix... you are working with any weird and wonderful organisms. While your samples are sterilizing in the autoclave...s possible that: The antibiotic broke down. You forgot to add the antibiotic. You added the antibiotic...

  3. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ... accuracy of your plasmid, please email help@addgene.org and include: The sequencing result The original...mutation is not an artifact, please email help@addgene.org with the sequence, trace file, and primer used...

  4. Kit Free RNA Extraction

    Type
    Protocol
    ...RNAse-free tube. The mixture separates into a bottom organic layer, an interphase layer, and a top, aqueous ...

  5. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...pLKO.1 containing the 1.9kb stuffer, visit www.addgene.org/10878/ . Description Vector Element U6 Human...resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium (TRC): www.broad.mit.edu...

  6. Protocol - How to Streak a Plate

    Type
    Protocol
    ...within a laboratory setting is important for organization, and it is recommended that you keep a standard...

  7. Video Library

    Type
    Protocol
    ... page . Please feel free to email us at help@addgene.org with any questions. Protocols Video protocol ...

  14. CRISPR Library Amplification

    Type
    Protocol
    ...DNA: guide RNAs targeting different genes in an organism's genome, for example. Amplification is usually...

  15. Lentivirus ddPCR Titration

    Type
    Protocol
    ...additional 10-fold dilution of the virus stock! Don’t forget to factor this into your calculations later (see...
Showing: 1 - 15 of 15 results