...kilobases. Nature Methods , 6(5), 343–345. https://doi.org/10.1038/nmeth.1318 (Link opens in a new window)...genome. Nature Methods , 7(11), 901–903. https://doi.org/10.1038/nmeth.1515 (Link opens in a new window)... Gibson Isothermal Assembly. bioRxiv . https://doi.org/10.1101/2020.06.14.150979 (Link opens in a new ...

... This will clear up any confusion later if your forget your bottle in the autoclave. Place the gel mix... you are working with any weird and wonderful organisms. While your samples are sterilizing in the autoclave...s possible that: The antibiotic broke down. You forgot to add the antibiotic. You added the antibiotic...

... accuracy of your plasmid, please email help@addgene.org and include: The sequencing result The original...mutation is not an artifact, please email help@addgene.org with the sequence, trace file, and primer used...

...RNAse-free tube. The mixture separates into a bottom organic layer, an interphase layer, and a top, aqueous ...

...pLKO.1 containing the 1.9kb stuffer, visit www.addgene.org/10878/ . Description Vector Element U6 Human...resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium (TRC): www.broad.mit.edu...

...within a laboratory setting is important for organization, and it is recommended that you keep a standard...

... page . Please feel free to email us at help@addgene.org with any questions. Protocols Video protocol ...

...own work. Please feel free to email us at help@addgene.org with any questions. Intro to the Lab Bench Introductory...

...is for labs that work with pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae...

...for virus purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly...

... precipitated protein particles Bottom phase - Organic phase (protein) Pipet the aqueous DNA layer and...

...duplicate Load 5 μL of each sample in duplicate. Do not forget to include a no template control ( NTC = master...

...standard curve or dilute the unknown sample (don’t forget to factor in that dilution when calculating the...

...DNA: guide RNAs targeting different genes in an organism's genome, for example. Amplification is usually...

...additional 10-fold dilution of the virus stock! Don’t forget to factor this into your calculations later (see...