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Showing: 1 - 13 of 13 results

  1. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...different serotype(s) for transduction of a given organ. Tissue Serotype Central nervous system AAV1, AAV2...administration, distributing widely throughout the organism to target large or diffused biological structures... of systemic rAAV vectors that can target whole organs or specific cell types of the CNS (e.g. AAV-PHP.eB...Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids Available at Addgene Viral Vectors... . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 (Link opens in a new...

  2. Lentiviral Vector Guide

    Type
    Guide
    ...safety. Resources and References Resources on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral...Current Gene Therapy , 5 (4), 387–398. https://doi.org/10.2174/1566523054546224 PMID: 16101513 Dull, T...Journal of Virology , 72 (11), 8463–8471. https://doi.org/10.1128/jvi.72.11.8463-8471.1998 PMID: 9765382 ...lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh, H. E. G...Biomedicine & Pharmacotherapy , 128 , 110276. https://doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836 Johnson...& Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm.2021.03.018 PMID: 33981779 Kim, ...& Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm.2020.10.012 PMID: 33768124 Marshall...

  3. Adenovirus Guide

    Type
    Guide
    ...safety. Resources and References Resources on addgene.org Adenoviral Plasmids Available at Addgene AdenoBuilder...Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372 (Link opens in a new...Transduction and Targeted Therapy, 6 (1), 53. https://doi.org/10.1038/s41392-021-00487-6 (Link opens in a new...vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020-60238-5 (Link opens in a new...Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080/21645515.2017.1383575 (Link opens in a ...Journal of Virology, 85 (9), 4222–4233. https://doi.org/10.1128/JVI.02407-10 (Link opens in a new window...Review of Vaccines, 14 (10), 1347–1357. https://doi.org/10.1586/14760584.2015.1077122 (Link opens in a ...

  4. Plan Your Experiment

    Type
    Guide
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit...Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732 Doman...Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224 Gilbert...and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932 Glass...Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932 Hanna...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...safety. Resources and References Resources on addgene.org Gamma-retroviral Plasmids Collection Viral Vectors...Journal of Virology , 88 (8), 4504–4513. https://doi.org/10.1128/jvi.00011-14 PMID: 24501411 Gilroy, K. ...genomics. PLoS ONE , 11 (4), e0154070. https://doi.org/10.1371/journal.pone.0154070 PMID: 27097319 LaFave...Nucleic Acids Research , 42 (7), 4257–4269. https://doi.org/10.1093/nar/gkt1399 PMID: 24464997 Maetzig, T.,...application. Viruses , 3 (6), 677–713. https://doi.org/10.3390/v3060677 PMID: 21994751 Mekkaoui, L., Tejerizo...& Clinical Development , 28 , 116–128. https://doi.org/10.1016/j.omtm.2022.12.006 PMID: 36620071 Mirow...GALV envelope. Viruses , 13 (8), 1471. https://doi.org/10.3390/v13081471 PMID: 34452336 Schnierle, B. ...

  6. Modular Cloning Guide

    Type
    Guide
    ...at 18 levels for rapid characterization in new organisms. Mobius Assembly Vector Toolkit Bacterial Expression...Toolbox Kits Bacterial Expression Thorsten Mascher , Georg Fritz Two sets of plasmids to generate constructs...reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial...Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering, TALEN Stephen Ekker...fusion proteins for use in many different model organisms. Diatom uLoop Assembly Kit Other, Yeast Expression...tags, and signal peptides, for use in a range of organisms including diatoms, yeast, plants and bacteria....

  7. Molecular Biology Reference

    Type
    Guide
    ...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...when trying to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would ...

  8. Chemogenetics Guide

    Type
    Guide
    ...that both KORD and hM3Dq can be used in the same organism to allow bidirectional control of neuronal activity...Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, Lieb A (2019). Olanzapine...

  9. Antibody Guide

    Type
    Guide
    ...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

  10. CRISPR Guide

    Type
    Guide
    ...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...

  11. Promoters

    Type
    Guide
    ...promoter region controls when and where in the organism your gene of interest is expressed. Summary Promoters...

  12. Sequencing Primers

    Type
    Guide
    ... sure what primer you need? Email us at help@addgene.org Addgene has used a number of primers for sanger...

  13. Optogenetics Guide

    Type
    Guide
    ...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...
Showing: 1 - 13 of 13 results