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...that both KORD and hM3Dq can be used in the same organism to allow bidirectional control of neuronal activity...Biology. Physiol Rev, 98 (1), 391–418. https://doi.org/10.1152/physrev.00009.2017 PMID: 29351511 Armbruster...Natl Acad Sci USA, 104 (12), 5163–5168. https://doi.org/10.1073/pnas.0700293104 PMID: 17360345 Berglund...Natl Acad Sci USA, 113 (3), E358–E367. https://doi.org/10.1073/pnas.1510899113 PMID: 26733686 Björefeldt...bioluminescence. Neurophotonics 11 (2), 021005. https://doi.org/10.1117/1.NPh.11.2.021005 PMID: 38450294 Coward...Proc Natl Acad Sci USA, 95 (1), 352–357. https://doi.org/10.1073/pnas.95.1.352 PMID: 9419379 Davies, M. ... Psychopharmacology, 178 (4), 451–460. https://doi.org/10.1007/s00213-004-2017-1 PMID: 15765260 Farrell...

...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...different serotype(s) for transduction of a given organ. Tissue Serotype Central nervous system AAV1, AAV2...administration, distributing widely throughout the organism to target large or diffused biological structures... of systemic rAAV vectors that can target whole organs or specific cell types of the CNS (e.g. AAV-PHP.eB...Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids Available at Addgene Viral Vectors... . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024 (Link opens in a new...

...safety. Resources and References Resources on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral...Current Gene Therapy , 5 (4), 387–398. https://doi.org/10.2174/1566523054546224 PMID: 16101513 Dull, T...Journal of Virology , 72 (11), 8463–8471. https://doi.org/10.1128/jvi.72.11.8463-8471.1998 PMID: 9765382 ...lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh, H. E. G...Biomedicine & Pharmacotherapy , 128 , 110276. https://doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836 Johnson...& Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm.2021.03.018 PMID: 33981779 Kim, ...& Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm.2020.10.012 PMID: 33768124 Marshall...

...safety. Resources and References Resources on addgene.org Adenoviral Plasmids Available at Addgene AdenoBuilder...Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372 (Link opens in a new...Transduction and Targeted Therapy, 6 (1), 53. https://doi.org/10.1038/s41392-021-00487-6 (Link opens in a new...vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020-60238-5 (Link opens in a new...Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080/21645515.2017.1383575 (Link opens in a ...Journal of Virology, 85 (9), 4222–4233. https://doi.org/10.1128/JVI.02407-10 (Link opens in a new window...Review of Vaccines, 14 (10), 1347–1357. https://doi.org/10.1586/14760584.2015.1077122 (Link opens in a ...

...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...activity. Nat Neurosci, 8 (9), 1263–1268. https://doi.org/10.1038/nn1525 PMID: 16116447 Chuong, A. S., Miri...rhodopsin. Nat Neurosci, 17 (8), 1123–1129. https://doi.org/10.1038/nn.3752 PMID: 24997763 Deisseroth, K., ...circuits. J Neurosci, 26 (41), 10380–10386. https://doi.org/10.1523/JNEUROSCI.3863-06.2006 PMID: 17035522 Emiliani...systems. Nat Rev Methods Primers, 2 , 55. https://doi.org/10.1038/s43586-022-00136-4 PMID: 37933248 Gradinaru... optogenetics. Cell, 141 (1), 154–165. https://doi.org/10.1016/j.cell.2010.02.037 PMID: 20303157 Han, ...temporal resolution. PloS One, 2 (3), e299. https://doi.org/10.1371/journal.pone.0000299 PMID: 17375185 Klapoetke...

...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit...Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732 Doman...Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224 Gilbert...and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932 Glass...Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932 Hanna...

...safety. Resources and References Resources on addgene.org Gamma-retroviral Plasmids Collection Viral Vectors...Journal of Virology , 88 (8), 4504–4513. https://doi.org/10.1128/jvi.00011-14 PMID: 24501411 Gilroy, K. ...genomics. PLoS ONE , 11 (4), e0154070. https://doi.org/10.1371/journal.pone.0154070 PMID: 27097319 LaFave...Nucleic Acids Research , 42 (7), 4257–4269. https://doi.org/10.1093/nar/gkt1399 PMID: 24464997 Maetzig, T.,...application. Viruses , 3 (6), 677–713. https://doi.org/10.3390/v3060677 PMID: 21994751 Mekkaoui, L., Tejerizo...& Clinical Development , 28 , 116–128. https://doi.org/10.1016/j.omtm.2022.12.006 PMID: 36620071 Mirow...GALV envelope. Viruses , 13 (8), 1471. https://doi.org/10.3390/v13081471 PMID: 34452336 Schnierle, B. ...

...at 18 levels for rapid characterization in new organisms. Mobius Assembly Vector Toolkit Bacterial Expression...Toolbox Kits Bacterial Expression Thorsten Mascher , Georg Fritz Two sets of plasmids to generate constructs...reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial...Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering, TALEN Stephen Ekker...fusion proteins for use in many different model organisms. Diatom uLoop Assembly Kit Other, Yeast Expression...tags, and signal peptides, for use in a range of organisms including diatoms, yeast, plants and bacteria....

... A, T, C, and G, respectively). The bases are organized into a right-handed, double-stranded helix. To...the instructions to make every protein within an organism. Before proteins can be translated, the DNA is...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and cultured human...

...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...

...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...

...promoter region controls when and where in the organism your gene of interest is expressed. Promoters ...

...best primer for your plasmid? Email us at help@addgene.org . For additional information on molecular biology...