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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 111 results for: org

Showing: 1 - 20 of 111 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...SP-dCas9-VPR transcriptional activator with Tet-On 3G George Church 87360 TLCV2 Lentiviral vector for tet-inducible...Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org/10.2174/1566523216666160524144041 (Link opens in...Natl Acad Sci U S A, 89 (12), 5547–5551. https://doi.org/10.1073/pnas.89.12.5547 (Link opens in a new window...cells . Science, 268 (5218), 1766–1769. https://doi.org/10.1126/science.7792603 (Link opens in a new window...range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link opens in a new window...transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04 (Link opens in a new window) ... expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10-81 (Link opens in a new window...

  2. Plan Your Experiment

    Type
    Collection
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit...Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732 Doman...Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224 Gilbert...and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932 Glass...Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932 Hanna...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    .... Nature Methods , 19 (11), 1371–1375. https://doi.org/10.1038/s41592-022-01638-5. PMID 36280721 (Link... . Molecular Systems Biology , 19 (8). https://doi.org/10.15252/msb.202110591. PMID 37477096 (Link opens...Nature Biotechnology , 36 (7), 638–644. https://doi.org/10.1038/nbt.4150. PMID 29889213 (Link opens in ...library . Biochemistry , 57 (31), 4717–4725. https://doi.org/10.1021/acs.biochem.8b00410. PMID 29920078 (Link...phosphorylation . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9130. PMID 26350500 (Link opens in...synthesis . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9168. PMID 26350765 (Link opens in...mitophagy . Molecular Cell , 60 (1), 7–20. https://doi.org/10.1016/j.molcel.2015.08.016. PMID 26365381 (Link...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    ...been established as a powerful vertebrate model organism for the study of genetics and developmental biology...Multicolor fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled ...screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected...assembly for the zebrafish genome. Zebrafish Model Organism Database (ZFIN) - A curated database of genetic... International Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research...aims to increase the use of zebrafish as a model organism and to foster advanced training of young scientists...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...specifically localize to cellular compartments or organelles. Addgene’s database includes many fluorescent...vs. in vivo, cytoplasmic vs. extracellular vs. organelle-localized). Browse or search the tables below ...and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(...sensors (green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA...Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging. ACS Chem Biol. 2015 Sep 18;...

  6. Worm Expression Resources

    Type
    Collection
    ... “the worm”) is widely used as a genetic model organism due to their rapid life cycle (3 days from egg...Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids that streamlines... Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate... related miniMos plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI...methods to engineer the genome of the genetic model organism C. elegans. Return to top Do you have suggestions...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  7. Genetic Code Expansion

    Type
    Collection
    ...RF1 function removed George Church 48999 C321 all TAG sites changes to UAG George Church 49018 C321.ΔA.exp...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA all TAG sites changes to UAG..., RF1 function removed, improved doubling time. George Church 98564 C321.Ub-UAG-sfGFP all TAG sites changes...function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all TAG sites... inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG codons ...codons. Deletion of serT, serU, prfA, and recA genes George Church 192872 rEcoli XpS All 321 UAG codons changed...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization... fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your...General Structures The cell contains a variety of organelles and subcellular structures each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  9. CRISPR References and Information

    Type
    Collection
    ...sequence or list of sequences. Developed by the George Church Lab and maintained by the Frederick National...Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed by the Jin-Soo Kim Lab . Cas-Designer...target site common to a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish...upload. Currently supports a wide selection of organisms. From the Database Center for Life Science (DBCLS...off-target binding. Currently supports a wide range of organims. Developed by the Michael Boutros Lab . CasFinder...Currently supports: mouse and human. Developed by the George Church Lab . CRISPR Optimal Target Finder (Link...

  10. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z (Link opens in a new...S. cerevisiae. PLoS One, 8 (7):e67902. https://doi.org/10.1371/journal.pone.0067902 (Link opens in a new...ammonium uptake. Nature, 446 (7132):195-8. https://doi.org/10.1038/nature05579 (Link opens in a new window...Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org/10.1002/yea.1130 (Link opens in a new window) PMID... cerevisiae. Microb Cell Fact, 12 :96. https://doi.org/10.1186/1475-2859-12-96 (Link opens in a new window...

  11. Microbiology Resources

    Type
    Collection
    ...these model organisms. See below for plasmid kits and other resources relevant to these organisms, or use ...depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome editing of...extensive fluorescent protein plasmid collection is organized by application and by color. External Resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  12. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Related Resources SGC: http://www.thesgc.org/ SGC Toronto: https://www.thesgc.org/scientists/groups/toronto...Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional...www.sgc.ox.ac.uk/ Protein Database (PDB): http://www.pdb.org Request Plasmids To order, Login or Register ...

  13. Distribution to Industry

    Type
    Collection
    ...account and affiliate it with your organization. If your organization has not ordered from Addgene in the...fax, or email. Pricing Addgene is a nonprofit organization dedicated to providing the scientific community...through your user account or by email to mta@addgene.org . After we review and approve the MTA documents...

  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Fluorescent Protein Fusions (Organized by Expression Species) Organism/Expression System Plasmids Mammalian...proteins with your gene of interest. These are organized By Color of the fluorescent protein and By Expression...Empty Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation and Emission...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource! Jump to Top...

  15. CRISPR Plasmids - Tagging

    Type
    Collection
    ...locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for... and a prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  16. MAPK Plasmids

    Type
    Collection
    ...email deposit@addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  17. CRISPR Plasmids and Resources

    Type
    Collection
    ...collections feature plasmids for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila... on our popular CRISPR 101 blog series, we've organized a comprehensive CRISPR resource for you to download...target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria...

  18. Synthetic Biology - Overview

    Type
    Collection
    ...Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins Tom Ellis ...plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  19. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases (AcpS ...previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson K. 2004...Chem. 273(35):22773-81. PMID:9712910 Vivero-Pol L, George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor...

  20. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... the expression of specific genes in cells or organisms. Each recombinase enzyme recognizes and binds ...sequence of the recombinase to align with your organism's preferred codon usage will improve expression...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 1 - 20 of 111 results