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...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...endonuclease. Nature , 513 (7519), 569–573. https://doi.org/10.1038/nature13579 PMID: 25079318 Bondy-Denomy... system. Nature , 493 (7432), 429–432. https://doi.org/10.1038/nature11723 PMID: 23242138 Bondy-Denomy...proteins. Nature , 526 (7571), 136–139. https://doi.org/10.1038/nature15254 PMID: 26416740 Bravo, J. P....CRISPR–Cas9. Nature , 603 (7900), 343–347. https://doi.org/10.1038/s41586-022-04470-1 PMID: 35236982 Bubeck...CRISPR–Cas9. Nature Methods , 15 (11), 924–927. https://doi.org/10.1038/s41592-018-0178-9 PMID: 30377362 Casini...

...SP-dCas9-VPR transcriptional activator with Tet-On 3G George Church 87360 TLCV2 Lentiviral vector for tet-inducible...Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org/10.2174/1566523216666160524144041 (Link opens in...Natl Acad Sci U S A, 89 (12), 5547–5551. https://doi.org/10.1073/pnas.89.12.5547 (Link opens in a new window...cells . Science, 268 (5218), 1766–1769. https://doi.org/10.1126/science.7792603 (Link opens in a new window...range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link opens in a new window...transregulators . BioTechniques, 37 (4), 546–550. https://doi.org/10.2144/04374BM04 (Link opens in a new window) ... expression . BMC Biotechnol, 10 , 81. https://doi.org/10.1186/1472-6750-10-81 (Link opens in a new window...

...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...in Molecular Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit...Screens. Cell , 167 (7), 1853–1866.e17. https://doi.org/10.1016/j.cell.2016.11.038 PMID: 27984732 Doman...Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/s41596-022-00724-4 PMID: 35941224 Gilbert...and activation. Cell , 159 (3), 647–661. https://doi.org/10.1016/j.cell.2014.09.029 PMID: 25307932 Glass...Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006 PMID: 25307932 Hanna...

.... Nature Methods , 19 (11), 1371–1375. https://doi.org/10.1038/s41592-022-01638-5. PMID 36280721 (Link... . Molecular Systems Biology , 19 (8). https://doi.org/10.15252/msb.202110591. PMID 37477096 (Link opens...Nature Biotechnology , 36 (7), 638–644. https://doi.org/10.1038/nbt.4150. PMID 29889213 (Link opens in ...library . Biochemistry , 57 (31), 4717–4725. https://doi.org/10.1021/acs.biochem.8b00410. PMID 29920078 (Link...phosphorylation . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9130. PMID 26350500 (Link opens in...synthesis . Nature Communications , 6 (1). https://doi.org/10.1038/ncomms9168. PMID 26350765 (Link opens in...mitophagy . Molecular Cell , 60 (1), 7–20. https://doi.org/10.1016/j.molcel.2015.08.016. PMID 26365381 (Link...

...RF1 function removed George Church 48999 C321 All TAG sites changed to TAA George Church 49018 C321.ΔA.exp...orthogonal pairs of aaRS and tRNA that work for one organism may not work for another. The orthogonal aaRS ...removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA All TAG sites changed to TAA..., RF1 function removed, improved doubling time. George Church 98564 C321.Ub-UAG-sfGFP All TAG sites changed...function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP All TAG sites... inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG codons ...codons. Deletion of serT, serU, prfA, and recA genes George Church 192872 rEcoli XpS All 321 UAG codons changed...

...been established as a powerful vertebrate model organism for the study of genetics and developmental biology...Multicolor fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled ...screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected...assembly for the zebrafish genome. Zebrafish Model Organism Database (ZFIN) - A curated database of genetic... International Zebrafish Society (IZFS) - An organization that promotes and advocates for zebrafish research...aims to increase the use of zebrafish as a model organism and to foster advanced training of young scientists...

...-2 . Nature medicine, 26 (4), 450–452. https://doi.org/10.1038/s41591-020-0820-9 (Link opens in a new ...virology, 82 (17), 8887–8890. https://doi.org/10.1128/JVI.00415-08 (Link opens in a new window...Journal of virology, 88 (2), 1293–1307. https://doi.org/10.1128/JVI.02202-13 (Link opens in a new window...Inhibitor . Cell, 181 (2), 271–280.e8. https://doi.org/10.1016/j.cell.2020.02.052 (Link opens in a new...journal of molecular sciences, 21 (6), 1947. https://doi.org/10.3390/ijms21061947 (Link opens in a new window...transduction and targeted therapy, 5 (1), 283. https://doi.org/10.1038/s41392-020-00426-x (Link opens in a new...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules...10 15 M -1 cm -1 nm 4 ). Values obtained from FPbase.org FRET calculator (Link opens in a new window) ...can be influenced by many factors, such as the organism or cell type, relative position and orientation...FRET pairs and biosensors can also incorporate organic fluorophores via HaloTag or SNAP-Tag . Read more...FRET Pairs . Sensors , 16 (9), 1488. doi: https://doi.org/10.3390/s16091488 (Link opens in a new window) ...Nature Methods , 16 (9), 815–829. doi: https://doi.org/10.1038/s41592-019-0530-8 (Link opens in a new ...Link opens in a new window) External Resources FPbase.org FRET calculator (Link opens in a new window) ...

...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...specifically localize to cellular compartments or organelles. Addgene’s database includes many fluorescent...vs. in vivo, cytoplasmic vs. extracellular vs. organelle-localized). Browse, sort, or search the tables... Vivo Imaging of Ca 2+ Dynamics in High-Ca 2+ Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(...sensors (green, red, or blue/green) Imaging intraorganellar Ca 2+ at subcellular resolution using CEPIA...Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn 2+ Imaging. ACS Chem Biol. 2015 Sep 18;10...

... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ...localization patterns for colocalization studies or organelle labeling. Plasmid...visualizing particular cellular structures or organelles, to observe cytoskeleton remodeling or blocked... fusion proteins that are targeted to various organelles or subcellular structures. To compare with a ...Structures Eukaryotic cells contain a variety of organelles and subcellular structures, each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... “the worm”) is widely used as a genetic model organism due to their rapid life cycle (3 days from egg...Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids that streamlines... Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate... related miniMos plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI...methods to engineer the genome of the genetic model organism C. elegans. Return to top Do you have suggestions...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...sequence or list of sequences. Developed by the George Church Lab and maintained by the Frederick National...Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed by the Jin-Soo Kim Lab . Cas-Designer...target site common to a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish...upload. Currently supports a wide selection of organisms. From the Database Center for Life Science (DBCLS...off-target binding. Currently supports a wide range of organims. Developed by the Michael Boutros Lab . CasFinder...Currently supports: mouse and human. Developed by the George Church Lab . CRISPR Optimal Target Finder (Link...

...in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z (Link opens in a new...S. cerevisiae. PLoS One, 8 (7):e67902. https://doi.org/10.1371/journal.pone.0067902 (Link opens in a new...ammonium uptake. Nature, 446 (7132):195-8. https://doi.org/10.1038/nature05579 (Link opens in a new window...Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org/10.1002/yea.1130 (Link opens in a new window) PMID... cerevisiae. Microb Cell Fact, 12 :96. https://doi.org/10.1186/1475-2859-12-96 (Link opens in a new window...

...these model organisms. See below for plasmid kits and other resources relevant to these organisms, or use ...depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome editing of...extensive fluorescent protein plasmid collection is organized by application and by color. External Resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...interactome. . Mol Syst Biol, 21 (6), 632-675. https://doi.org/10.1038/s44320-025-00109-1 (Link opens in a new...carriers. . Mol Syst Biol, 21 (6), 599-631. https://doi.org/10.1038/s44320-025-00108-2 (Link opens in a new.... Nat Rev Drug Discov, 19 (7):429-430. https://doi.org/10.1038/d41573-020-00056-6 (Link opens in a new...superfamily. . Mol Syst Biol, 21 (6), 560-598. https://doi.org/10.1038/s44320-025-00106-4 (Link opens in a new...superfamily. . Mol Syst Biol, 21 (6), 531-559. https://doi.org/10.1038/s44320-025-00105-5 (Link opens in a new...

..., AmpR Related Resources SGC: www.thesgc.org SGC Toronto: www.thesgc.org/scientists/groups/toronto Protein...Structural Genomics Consortium is a not-for-profit organization that aims to determine the three dimensional...Protein Database (PDB): www.pdb.org Request Plasmids To order, Login or Register a new account. Add the...

...account and affiliate it with your organization. If your organization has not ordered from Addgene in the...fax, or email. Pricing Addgene is a nonprofit organization dedicated to providing the scientific community...through your user account or by email to mta@addgene.org . After we review and approve the MTA documents...

...locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for... and a prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...cell types. Cell , 188 (11), 3045–3064. https://doi.org/10.1016/j.cell.2025.05.002 PMID: 40403729 Graybuck...labeling. Neuron , 109 (9), 1449–1449. https://doi.org/10.1016/j.neuron.2021.03.011 PMID: 33789083 Hunker...circuits. Neuron , 113 (10), 1507–1524. https://doi.org/10.1016/j.neuron.2025.04.035 PMID: 40403704 Mich...neocortex. Cell Reports , 34 (13), 108754. https://doi.org/10.1016/j.celrep.2021.108754 PMID: 33789096 Additional...

...upregulating gene expression in various model organisms....CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...