We narrowed to 30,796 results for: Ide

1. p-miR-Report + WT miR-181 BS in GATA6 3UTR

   Plasmid

   #22794

   Depositor

   Xin Wang

   Article

   Ji et al Hepatology. 2009 Aug . 50(2):472-80.

   Insert

   Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
   

   Use

   Luciferase

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

2. p-miR-Report + MT miR-181 BS in GATA6 3UTR

   Plasmid

   #22795

   Depositor

   Xin Wang

   Article

   Ji et al Hepatology. 2009 Aug . 50(2):472-80.

   Insert

   Mutant miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
   

   Use

   Luciferase

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

   Plasmid

   #176589

   Purpose

   Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Chesapeake Bay) locus
   

   Expression

   Bacterial

   Mutation

   Truncated CRISPR array to two direct repeats, rep…

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM33_Nsp7

   Plasmid

   #156476

   Purpose

   Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_Nsp8

   Plasmid

   #156477

   Purpose

   Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM33_Nsp3b_ADRP

   Plasmid

   #156468

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3b_ADRP (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCCL-PGK-SPdCas9-BFP-DNMT3B

   Plasmid

   #66820

   Purpose

   dCas9 fused to BFP and the human DNMT3B catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
   

   Tags

   dSpCas9-BFP-DNMT3B

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-FLAG-SpCas9-HF1-plus

   Plasmid

   #126775

   Purpose

   Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A; Q695A; Q926A; amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-RAB10 K154A

    Plasmid

    #130886

    Purpose

    Expresses human RAB10 K154A mutant with N-terminal GFP tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    RAB10 (RAB10 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K154A

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid

    #127483

    Purpose

    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    One nucleotide inserted between lambda CI and LGF…

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM33_Nsp3a_Ub1

    Plasmid

    #156467

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3a_Ub1 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM33_Orf10

    Plasmid

    #156486

    Purpose

    Bacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Orf10 (ORF10 Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

    Plasmid

    #71219

    Purpose

    dCas9 fused to the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3B(E697A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-miR-Report + WT miR-181 BS in CDX2 3UTR

    Plasmid

    #22792

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Wild-type miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pETM33_Nsp3c_SUD-N

    Plasmid

    #156469

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-N (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM33_Nsp3c_SUD-M

    Plasmid

    #156470

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-M (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2-mkgDUX4mal*mqg*

    Plasmid

    #21174

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    First point mutation at nucleotide 5114 (numberin…

    Available Since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pLL6 sgRNA(MS2)-MS2-hA3a D131E

    Plasmid

    #112132

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131E

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131E mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D131E mutant in hA3a

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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