We narrowed to 8,621 results for: Ott

1. EGFP-Rab32b

   Plasmid

   #140885

   Purpose

   Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab32b (rab32b Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmScarlet-H_NES_C1

   Plasmid

   #85061

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-mH6-Cas12c1

   Plasmid

   #120872

   Purpose

   Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HisTEV-cDAXX

   Plasmid

   #197012

   Purpose

   E. coli expression vector

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   DAXX
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-mH6-Cas12c2

   Plasmid

   #120873

   Purpose

   Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c2
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-mH6-Cas12g1

   Plasmid

   #120879

   Purpose

   Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12g1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPP381

   Plasmid

   #158798

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   RuvC-inactivating mutation D394A

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFS478

   Plasmid

   #89066

   Purpose

   KanMX Z3EV promoter cassette

   Depositor

   Nick Rhind

   Article

   Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

   Insert

   Z3EV promoter cassette
   

   Use

   Yeast integration vector

   Tags

   Expression

   Mutation

   yeast Gal1 promoter modified with multiple Zif268…

   Promoter

   Z3EV promoter

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-DeltaJunD

    Plasmid

    #68547

    Purpose

    AAV expression of DeltaJunD

    Depositor

    Eric Nestler

    Article

    Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

    Insert

    DeltaJunD-IRES-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    truncated splice variant

    Promoter

    CMV

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pAAV.hSyn-FLEX.iGABASnFR.F102G

    Plasmid

    #112164

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Has Service

    AAV1

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102G

    Promoter

    Synapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-smyhc1

    Plasmid

    #126748

    Purpose

    Zebrafish slow myosin heavy chain promoter. Clone 667

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    smyhc1 (smyhc1 Zebrafish)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD/HisB-PAmCherry1

    Plasmid

    #31931

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    araB

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTfR-PAmCherry1

    Plasmid

    #31948

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    transferrin receptor (TfR)

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-PE2-N

    Plasmid

    #164909

    Purpose

    Expresses the N-terminal split-intein fragment of PE2

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    PE2-N
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Lyn-PlcR

    Plasmid

    #181830

    Purpose

    Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-PlcR
    

    Use

    Tags

    ECFP, EYFP, and N-terminal targeting sequence fro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV2_HLF-ZP

    Plasmid

    #175035

    Purpose

    HLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon

    Depositor

    Guy Sauvageau

    Article

    Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

    Insert

    HLF ires ZsGreen P2A Puro
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 iTEV

    Plasmid

    #64277

    Purpose

    Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Infrared fluorescent tobacco etch virus protease sensor (iTEV)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

    Plasmid

    #200277

    Purpose

    Characterisation of the sialic acid-responsive circuit with fluorescent reporter

    Depositor

    Matthew Chang

    Article

    Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

    Inserts

    CadC
    NanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPP537

    Plasmid

    #158803

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    2xSV40NLS-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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