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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,279 results for: Vars

Showing: 3281 - 3300 of 11279 results

  10. TEG +Q111T

    Plasmid
    #187435
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al bioRxiv 2021.11.29.470343
    Inserts
    ATP1A1
    ATP1B1
    Use
    Tags
    Expression
    Insect
    Mutation
    changed glutamine at 117 to threonine
    Promoter
    PH and p10
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    187435-plasmid-map-sequence-id-395111

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  12. pGADT7-αC

    Plasmid
    #197650
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-2 αC
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Ser677T substitution
    Promoter
    ADH1
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197650-plasmid-map-sequence-id-388876

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  13. BPTI-21

    Plasmid
    #195058
    Purpose
    simplyfied amino acid sequence by alanine scanning
    Depositor
    Peter Kim, Yutaka Kuroda
    Article
    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d
    Insert
    bovine pancreatic trypsin inhibitor
    Use
    Tags
    His tag and trpΔLE leader sequences
    Expression
    Bacterial
    Mutation
    21 amino acids are Alanine out of 58 amino acids
    Promoter
    T7 promotor
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195058-plasmid-map-sequence-id-388061

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  14. pCRISPR3_BC2

    Plasmid
    #191857
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    191857-plasmid-map-sequence-id-381604

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Showing: 3281 - 3300 of 11279 results