We narrowed to 4,191 results for: Pet2

1. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SpyTag003-mKate2

   Plasmid

   #133452

   Purpose

   Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mKate2
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NW9

   Plasmid

   #133442

   Purpose

   Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NW11

   Plasmid

   #133443

   Purpose

   Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NW30

   Plasmid

   #133444

   Purpose

   Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW30
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SpyTag003-sfGFP

   Plasmid

   #133454

   Purpose

   Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-sfGFP
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Use

   Tags

   C-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LM627

   Plasmid

   #191551

   Purpose

   LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

   Depositor

   David Baker

   Article

   Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

   Insert

   ccdb
   

   Use

   Tags

   SNAC-tag, HIS-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LM670

   Plasmid

   #191552

   Purpose

   LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

   Depositor

   David Baker

   Article

   Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

   Insert

   ccdb
   

   Use

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ecoli cpGFP

    Plasmid

    #215713

    Purpose

    Expresses cpGFP in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    circularly permuted GFP

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJBL715

    Plasmid

    #140385

    Purpose

    Expresses MphR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-MphR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJBL723

    Plasmid

    #140393

    Purpose

    Expresses HucR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-HucR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL713

    Plasmid

    #140383

    Purpose

    Expresses CtcS protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CtcS-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FnR9-10

    Plasmid

    #182567

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Use

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Human USP30 (64-502, construct 8, MG-26-21)

    Plasmid

    #110745

    Purpose

    Bacterial expression of human USP30 (construct 8)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    F348D, M350D, I353E + insertion deletion: 64-357;…

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOlyA(WT)

    Plasmid

    #132391

    Purpose

    Ostreolysin A for sensing sphingomyelin/cholesterol complexes

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A wild-type
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, C94S, S151C

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDR119

    Plasmid

    #13332

    Depositor

    Martin Gellert

    Article

    Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

    Insert

    DNA ligase IV (LIG4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only