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Plasmid#64732PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNAR2
UseTagsCerulean3, N-terminal 27 amino acids from CYP450,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
PresentER-MSIIFFLPL (GFP)
Plasmid#102946PurposePresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)DepositorInsertMSIIFFLPL (Serpinf1 Synthetic, Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag
Plasmid#179715PurposeLentiviral expression of mouse Syt7 with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS-HsProm1s1-StayGold
Plasmid#211333PurposeVector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)DepositorInsertProminin 1 (PROM1 Human)
UseAAVTagsStayGoldExpressionMutationPromoterEF1aAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-CaNAR2
Plasmid#64730PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertLyn-CaNAR2
UseTagsCerulean3, N-terminal targeting sequence from Lyn…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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ABHD16A-mNG
Plasmid#195157Purposemammalian expression of ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
UseTagsmNeonGreenExpressionMammalianMutationNonePromoterCMVAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFE-Reu-FI-prk
Plasmid#162696PurposeExpresses R. eutropha Form IC rubisco and S. elongatus PrkDepositorInsertR. eutropha CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
σ1-mCherry
Plasmid#186578PurposeExpression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherryDepositorInsertAP1S1 (AP1S1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only