We narrowed to 25,959 results for: Vit;

1. pcDNA3-ExRai-AMPKAR(T/A)

   Plasmid

   #192447

   Purpose

   Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR(T/A)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Thr 6 in AMPK substrate mutated to Ala.

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. shh10

   Plasmid

   #64867

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

   Insert

   shh10 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   I319V, N451D, D532N

   Promoter

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLemir9-124

   Plasmid

   #31779

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. myc-hIFITM3-delta59-68

   Plasmid

   #104361

   Purpose

   Expresses human IFITM3-delta59-68 with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

   Insert

   IFITM3 (IFITM3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   deleted amino acids 59 through 68

   Promoter

   CMV

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx-RhoA FLARE.sc Biosensor WT

   Plasmid

   #12150

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor (RHOA Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-miRE_shBRD9_561-SV40-GFP

   Plasmid

   #75139

   Purpose

   Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   shBRD9_561
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET11a-SUMO1

   Plasmid

   #53138

   Purpose

   E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mature form (amino acids 1-97)

   Promoter

   T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. wtBxb1_IVT

   Plasmid

   #222340

   Purpose

   Plasmid for wild type Bxb1 mRNA in vitro transcription template

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized wtBxb1
   

   Use

   Template for in vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. 408 pSG5L HA E2F1

   Plasmid

   #10736

   Depositor

   William Sellers

   Article

   Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MT363

    Plasmid

    #65858

    Purpose

    The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

    Depositor

    Detlef Weigel

    Article

    Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

    Insert

    nahG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPLICS PM-ER Long P2A

    Plasmid

    #164111

    Purpose

    Detect the long Plasma membrane-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP PM-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLL7.0: Venus-iLID-CAAX (from KRas4B)

    Plasmid

    #60411

    Purpose

    Mammalian Expression of Venus-iLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUW-Sox10

    Plasmid

    #36978

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Sox10 (SOX10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T-1-3xFLAG-HSP27

    Plasmid

    #129229

    Purpose

    Encodes FLAG tagged human HSP27 for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    HSP27 (HSPB1 Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCW-CCNE1-HA-puro

    Plasmid

    #212669

    Purpose

    Express tagged cyclin E1

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SPLICS Mt-ER- PM Long P2A

    Plasmid

    #164109

    Purpose

    Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM-Mt-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 FlipGFP(Mpro) T2A mCherry

    Plasmid

    #163078

    Purpose

    Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

    Depositor

    Xiaokun Shu

    Article

    Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

    Insert

    FlipGFP-based Mpro activity reporter and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC-EHER

    Plasmid

    #53262

    Purpose

    Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, idi, crtX, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Ub KO.G76V

    Plasmid

    #11932

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin KO.G76V (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Conjugation deficient mutant. All 7 lysines of ub…

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-mas-GRK2RH-Nluc

    Plasmid

    #158605

    Purpose

    Plasmid expressing GRK2(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Flag Nprl2 pRK5

    Plasmid

    #46333

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP‐RNASEH2A

    Plasmid

    #108700

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2A (RNASEH2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEMHE-mEGFP-mTrim21

    Plasmid

    #105519

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Use

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR-Hsp-eGFP-PGK-mCherry

    Plasmid

    #191862

    Purpose

    Heat inducible eGFP reporter with constitutive mCherry marker

    Depositor

    Yingxiao Wang

    Article

    Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

    Inserts

    eGFP
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSPA7 promoter and PGK promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

    Plasmid

    #219782

    Purpose

    CaST-IRES for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid

    #83485

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLR5-CAG-TetR_W43F-3xmNG

    Plasmid

    #174880

    Purpose

    PiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreen

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    TetR(W43F)-3xmNeonGreen
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Yellow Cameleon-Nano15/pBig

    Plasmid

    #51962

    Purpose

    Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SPLICS PM-ER Short P2A

    Plasmid

    #164112

    Purpose

    Detect the short Plasma membrane-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP PM-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA-3xflagGREB1a

    Plasmid

    #112221

    Purpose

    mammalian expression of flag tagged GREB1a

    Depositor

    Craig Burd

    Article

    Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

    Insert

    GREB1a (GREB1 Human)
    

    Use

    Tags

    3x flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1-hRARα.hRXRα

    Plasmid

    #135411

    Purpose

    Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Inserts

    Human retinoic acid receptor-alpha
    Human retinoid X receptor-alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SV40

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. L4440_BioBrick-SCR-sgRNA-A

    Plasmid

    #177811

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    Scrambled sgRNA targeting sequences
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCS2+ Lifeact-mCherry

    Plasmid

    #185408

    Purpose

    Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    lifeact
    

    Use

    In vitro synthesis of mrna

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP‐RNASEH2B

    Plasmid

    #108697

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2B (RNASEH2B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SPLICS Po-ER Short P2A

    Plasmid

    #164114

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pdestMB14_sur5p-dCas9-VP64

    Plasmid

    #177788

    Purpose

    Overexpression of dCas9-VP64 in C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    dCas9-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SPLICS Po-ER Long P2A

    Plasmid

    #164113

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-BE4-PpAPOBEC1 R33A

    Plasmid

    #138345

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1 R33A

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1 R33A
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Change of Arg 33 to Ala.

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LL - hOCT4i -1

    Plasmid

    #12198

    Depositor

    George Daley

    Article

    Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

    Insert

    OCT4 - RNAi (POU5F1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge