...Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity Regulation More Fluorescent...these fluorescent proteins to modulate biological activity. Early tools allow scientists to regulate transcription...being developed to regulate a variety of other activities. By using GFP as a scaffold, scientists can design...See Plasmids Dronpa Optical control of protein activity Michael Lin See Plasmids Do you have suggestions...

...However, if we think of creativity as a form of innovation, it’s clear that creativity is absolutely a transferable... 2: Blugene likes to spark creativity in out on the open sea Creativity comes in many forms. Whether...highlighting an oft-underlooked transferable skill: creativity. I’ve heard quite a few scientists state that...transferable skill developed during PhD training. Creativity is required to develop new projects and test ... study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted an experiment in...dissenting opinions is to foster a culture of inclusivity. Work with and value people who think differently...training as a scientist has been building your creativity all along. In my own career, my team has used...

...there are more and more molecular biology-based activities making their way into high school classrooms,...transforming cells with green fluorescent protein, these activities are limited to those schools fortunate enough... of low-cost, easy-to-use educational biology activities – that any school could afford – led me to develop..., as each one is as cheap as 10 cents, making activities designed with FD-CF reactions affordable to many... accompanying curriculum to see if any of the activities fit your lesson plans. If you are an instructor...your research ideas into hands-on educational activities using the BioBits platform, please get in touch...

... for pathways that drive resistance to drugs in vitro, and they’ve shown that it works. But, he says that...

...insertions require complicated, often costly in vitro assembly methods. A new method, REPLACR-mutagenesis...mutagenesis methods Current SDM methods rely on in vitro assembly. To make a point mutation, you design two...Trehan et al. wondered if, instead of relying on in vitro assembly, they could make the E. coli do the work...since you skip the steps of gel purification and in vitro recombination. Across all modifications described...Reactions 1 1 1 per fragment 1 per fragment In vitro Assembly No No Yes Yes DpnI Digestion Yes Yes ...

...doing work outside of the lab. Extracurricular activities don't have to be ‘extra’ Today’s graduate and...of professions is to engage in extracurricular activities (EA). EAs can: Introduce new perspectives in ...outside of the academic world Participating in activities outside of the lab expands your professional ...! Taking action: Examples of extracurricular activities The table below lists potential EAs and which...within and beyond academia. Extracurricular Activities Professional Skills Open source classes (e.... interest in a different field Outreach activities (e.g. Volunteer at a local high school science...

...channelrhodopsin’s photocurrent strength, wavelength sensitivity, and off-kinetics.   This model was then used... measured photocurrent amplitude, wavelength sensitivity, and off-kinetics correlated with the model’s...experiments determined that ChRger2’s improved light sensitivity and higher-photocurrent properties when paired...

...experiment. Sensitivity Cas9 sensitivity is often closely related to specificity. Sensitivity is a measure...measure of off-target activity (left) while sensitivity is the measure of on-target activity (right). Cas9...by calculating the on-target activity divided by the off-target activity. Figure 2: Specificity...balancing specificity and sensitivity. Wild-type Cas9 exhibits high on-target activity, but also high off-target...consider: site accessibility, specificity, and sensitivity. Site accessibility Site accessibility is a measure...Specificity is the assessment of the off-target activity of Cas9. This can occur when Cas9 cleaves a site...measure of the on-target activity of the Cas9 nuclease. This can be determined by measuring the indel frequency...

...researchers to activate transcription or Cre recombinase activity only in the presence of GFP. These systems, known...

...kinase activity at the single-cell level can aid in drawing connections between signaling activity and cell...following phosphorylation bNLS activity is suppressed and NES activity is enhanced, leading to cytoplasmic...kinase activity.  Advantages of KTRs When compared to FRET reporters for measuring kinase activity, KTRs... method for monitoring live single-cell kinase activity is FRET, but FRET reporters are challenging to...localization serves as a proxy measurement of kinase activity. The key advantage of KTRs is that they are easy...together. Challenging  to Design: FRET reporter activity depends on both the distance and the orientation...FRET reporters. First, KTRs monitor global kinase activity that occurs in the cytoplasm and/or nucleus, while...

...Fisher 21028 Protein G GraviTrap columns, Cytiva 28-9852-55 or rProtein A GraviTrap columns, Cytiva 28-9852...and mix well. Uncap the Protein G Gravitrap or rProtein A Gravitrap columns and gently pour off the ethanol...Buffer Reservoirs to the top of the Gravitrap column. Pro-Tip Gravitrap columns have a finite binding capacity...the Gravitrap column to a clamp on a clamp stand. Use scissors to cut the bottom of the Gravitrap column...the Gravitrap columns at the bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap column...and diluted cell culture supernatant into the Gravitrap column. Collect the flow through in a 250 mL bottle...

... 1mm thick, 10-well, Invitrogen NP0321BOX 20X MOPS SDS running buffer, Invitrogen NP0001 4X NuPage sample...buffer, Invitrogen NP0007 10X NuPage sample reducing agent (500 mM dithiothreitol, DTT), Invitrogen NP0009...NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips, 10 µL, VWR 76322-132 Pipette...

...serum-free media Invitrogen: #31985 Dulbecco’s Modified Eagle Medium (DMEM) Invitrogen: #11995 Fetal Bovine...Bovine Serum (FBS) Invitrogen: #16000 Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene tubes... buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen...homologous recombination of unstable regions, such as Invitrogen Stbl3™ or NEB Stable cells. This will ensure ...

...exonuclease function of the enzyme and shift its activity back to polymerase. In our case, dGTP will be ...protocol), causing the enzyme to perform exonuclease activity until the first "G" in the sequence. Because the...proceeding, as they will interfere with the exonuclease activity of T4 Pol in the following step. This may be accomplished...

... However, keep in mind that restriction enzyme activity is determined under ideal conditions with very...their recognition sites. This is due to "Star Activity" and can happen for a variety of reasons, including...opens in a new window) NEB's website about star activity . If you are digesting a large number of plasmids...

...joined in a single isothermal reaction. With the activities of three different enzymes, the product of a ...ssDNA ends from the ssDNA-specific endonuclease activity of T5 Exonuclease and furthermore reduces secondary...

...window) Qiagen , (Link opens in a new window) Invitrogen , and (Link opens in a new window) Promega sell...

...standard Polymerase Chain Reaction (PCR) is an in vitro method that allows a single, short region of a DNA...

...cell line used, this antibiotic selection may be a vital step for obtaining a population of cells that have...

...signal the presence of specific biomolecules, the activity of specific genes and cellular processes, or other...differ from the original characterization (e.g. in vitro vs. in vivo , cytoplasmic vs. extracellular vs. ...) High-performance calcium sensors for imaging activity in neuronal populations and microcompartments....GCaMP6f/m/s calcium sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive...Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul 18;499(7458):295-300. Douglas ...expressing GCaMP6 and mRuby2 for imaging neural activity (GCaMP6f/m/s) (Constitutive or Cre-dependent) ...Optimization of a GCaMP calcium indicator for neural activity imaging. J Neurosci. 2012 Oct 3;32(40):13819-40...