We narrowed to 51 results for: Vit;

...Expression Species PI 14474 pRU1161 Promoter activity GUS activity and fluorescence (mRFP1) Gram-negative bacteria...bacteria Philip Poole 14473 pRU1156 Promoter activity GUS activity and fluorescence (GFPmut3.1) Gram-negative...Promoter activity Fluorescence (GFP+) Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity Fluorescence...Promoter activity Fluorescence (mCherry) Mycobacterium sp. Tanya Parish 24657 pASTA3 Promoter activity Fluorescence...Purification To study the function, structure, and activity of a protein it is generally necessary to purify...engineered strain for a B2H system used to screen the activity of arrays of zinc finger nucleases. Addgene Blog...Jessica Seeliger 202406 pJYP1 Riboswitch Cobalamin (vitamin B12) Escherichia coli Remy Loris 47663 pAC-EsaR-I70V...

...signal the presence of specific biomolecules, the activity of specific genes and cellular processes, or other...differ from the original characterization (e.g. in vitro vs. in vivo, cytoplasmic vs. extracellular vs. organelle-localized...) High-performance calcium sensors for imaging activity in neuronal populations and microcompartments....GCaMP6f/m/s calcium sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive...Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul 18;499(7458):295-300. Douglas ...Optimization of a GCaMP calcium indicator for neural activity imaging. J Neurosci. 2012 Oct 3;32(40):13819-40...genetically encoded calcium sensor Imaging neural activity in worms, flies and mice with improved GCaMP calcium...

...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV ...expression systems . ID Name Expression System Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven, constitutive...used for viral TRAP). ID Name Expression System Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven...of pre-synaptic mGRASP-mCerulean for synaptic connectivity mapping. 5 Jinhyun Kim 34912 paavCAG-post-mGRASP...of post-synaptic mGRASP-dTomato for synaptic connectivity mapping. 5 Jinhyun Kim Tools for Cell Ablation...used for cell ablation. ID Name Expression System Activity Serotype PI 45580 pAAV-flex-taCasp3-TEVp EF1a-...targeted genome editing. ID Name Expression System Activity Serotype PI 61592 pX600-AAV-CMV::NLS-SaCas9-NLS...

...proteins) Many base editors fused to Cas9 exert their activity within a very narrow window proximal to the PAM...essential protein domain could abolish protein activity and essentially function as a “knockout,” but ...gRNAs Based On Predicted On-Target and Off-Target Activity Another important factor to keep in mind when ...is the balance between on-target and off-target activity. In a perfect world, your gRNA sequence would ...with higher fidelity. In addition to off-target activity, it is also important to consider factors that...cleavage of the desired target sequence, or on-target activity. Two gRNA targeting sequences with 100% homology...sequence and your genomic target may reduce the gRNA activity. Be sure to sequence the genomic region you intend...

...SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease activity SpRY — NRN/NYN, where R ...nuclease activity Cas9 enzymes will likely improve as more key residues for specificity and activity are discovered...select for gRNAs with high on-target activity and low off-target activity. Knockout libraries often target...engineered to increase specificity and nuclease activity available. Engineered Cas9s have also led to reduced...off-target effects Sniper-Cas9 — less off-target activity; compatible with truncated gRNAs to increase specificity...about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk of off-target cutting or...(Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages and other...

...Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI Browse Glypican Antibodies.... ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI Return to top Synaptic...neurotransmission, synapse formation, neural connectivity, plasticity and neurological disorders. The ...Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications PI Return to top Integrins...Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Return to top Browse.... ID Recombinant Antibody Description Isotype Reactivity Recommended Applications Return to top Browse...

...quality control, including AAV titration by ddPCR, in vitro and (when possible) in vivo testing, and full sequencing...chloride, 20 mM Tris at pH 8.0) Suitable for in vitro as well as in vivo delivery where the vector is ...by the physiologic body fluids, such as blood, vitreous, CSF, etc. Not suitable as a buffer for vectors...enhanced transduction of the retina following intravitreal injection in mice and marmosets as compared ...enhanced transduction of the retina following intravitreal injection in mice and macaque. This serotype... citations include: Scalabrino, et al. 2015. Intravitreal delivery of a novel AAV vector targets ON bipolar...

...rtTA variants with lower background activity and greater sensitivity to doxycycline (Zhou et al., 2006)...components of these systems for better efficiency, sensitivity, and control of expression. These include: Tet-On...rtTA-Advanced, also known as rtTA2 S -M2) with greater sensitivity to doxycycline and three copies of a minimal ...transient or stable) may depend on the desired sensitivity, level of induction, or amount of background ...activators: novel mutations yield expanded range and sensitivity . Proc Natl Acad Sci U S A, 97 (14), 7963–7968...

...promoter. RCIscript-GoldyTALEN is designed for in vitro synthesis of TALEN mRNAs. Both 5’ and 3’ Xenopus...the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter. Truncations...BioSciences (N153AA, C63AA) and showed robust cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo... assay, which enables the evaluation of TALEN activities in cultured cells. Golden Gate TALEN 2.0 47388... included for generating TALEN mRNAs through in vitro transcription as an alternative. Based on transient...

...the antigen sequence, and cross-species reactivity. Reactivity is based on validation data from Addgene...Recombinant Antibody Description Source Species Isotype Reactivity Recommended Applications PI Return to Top Do ...

...using these opsins as a tool to control neuronal activity, scientists generally attach a fluorescent protein...protein interactions, dimerization, localization or activity of many different types of proteins. For a full...that use light to control a protein or cellular activity. For example, search "Cdc42", "transcription",...

...chemogenetics AAV to chemically induce neuronal activity in specific cell types. See our Chemogenetics ...mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent...Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361...

...that have been used for measuring viral entry and activity in neutralization assays and links to popular ... cleaves hACE2, potentially increasing viral infectivity (Heurich et al., 2014). PCP4 plasmids - (Purkinje... nucleic acids SARS-CoV-2 main protease (Mpro) activity reporters Reverse transcriptase, protease, and...

...promoters. These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5...switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent Flp-dependent Cre and Flp-dependent...Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive...

...(~25 kD), but tend to have lower affinity and longevity. Single-domain antibodies - Single-domain antibodies...monoclonal antibodies tend to have less cross-reactivity and give lower background signals than polyclonal...interest is knocked down or out, and antibody reactivity is compared between the wild-type and the knockdown...cell line with low or no expression; antibody reactivity is then compared between the knock-in cell line...

...CRISPRko/a/i sgRNA sequences to maximize on-target activity for selected target(s). Uses human, mouse, and...CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more. CasPEDIA...107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized...

... Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics is typically...fluorescent protein Blue-wavelength fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent...Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV CAG ChR2...

... the emission of photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching...screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter...G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR : BRET biosensor for live imaging of mTOR...

...could be programmed for targeted DNA cleavage in vitro. In 2013, Cong et al. and Mali et al. described ...gRNAs: Truncated gRNAs display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9...eSpCas9, and HypaCas9 display very low off-target activity due to rationally designed mutations. Researchers...

... that can be used for measuring infectivity and neutralizing activity are listed below: ID Plasmid Description...