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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 27,843 results for: REV

Showing: 8351 - 8400 of 27843 results

  13. pDONR223 LC3B2 no-stop

    Plasmid
    #123207
    Purpose
    Gateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal tagging
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    Use
    Gateway entry vector / entry clone
    Tags
    Expression
    Mutation
    Stop codon removed
    Promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123207-plasmid-map-sequence-id-244469

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  28. Spt2-K166R-GFP

    Plasmid
    #115573
    Purpose
    Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    SPT2
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    Lysine 166 mutated to Arginine
    Promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  30. Hsh155 N4A 3xFLAG

    Plasmid
    #111975
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 N4A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    T178A R181A R182A R186A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111975-plasmid-map-sequence-id-213742

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  31. Hsh155 K818A 3xFLAG

    Plasmid
    #111982
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. Lethal
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 K818A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    K818A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111982-plasmid-map-sequence-id-213737

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  32. Hsh155 R775A 3xFLAG

    Plasmid
    #111977
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. Lethal
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 R775A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    R775A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111977-plasmid-map-sequence-id-213729

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  33. Hsh155 R778A 3xFLAG

    Plasmid
    #111979
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. Lethal
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 R778A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    R778A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111979-plasmid-map-sequence-id-213728

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  36. pTH861-gst-optec P210T

    Plasmid
    #89595
    Purpose
    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed proline 210 to threonine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89595-plasmid-map-sequence-id-206078

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  37. pTH846-gst-dissc

    Plasmid
    #96907
    Purpose
    Plasmid for yeast expression of GST encoded by non-preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon dis-optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96907-plasmid-map-sequence-id-206054

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  38. pTH860-gst-optec A180Q

    Plasmid
    #89594
    Purpose
    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed alanine 180 to glutamine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89594-plasmid-map-sequence-id-206044

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  39. pTH858-gst-optec V156E

    Plasmid
    #89592
    Purpose
    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed valine 156 to glutamic acid
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89592-plasmid-map-sequence-id-206041

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  40. pTH859-gst-optec Y172M

    Plasmid
    #89593
    Purpose
    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed tyrosine 172 to methionine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89593-plasmid-map-sequence-id-206040

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Showing: 8351 - 8400 of 27843 results