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We narrowed to 741 results for: REV

Showing: 1 - 20 of 741 results

  1. Lentivirus Plasmids

    Type
    Collection
    ...along with a plasmid encoding rev . Didier Trono 12253 pRSV-Rev 3rd Contains rev . Can be used with 3rd generation...Expresses gag-pol and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd Contains gag , pol , rev , tat , vif , and...Didier Trono 12260 psPAX2 2nd Contains gag , pol , rev , and tat . Can be used with 2nd and 3rd generation...Trono 12263 pCMV delta R8.2 2nd Contains gag , pol , rev , and tat . Can be used with 2nd and 3rd generation...17531 pCD/NL-BH*DDD 2nd Contains gag-pol , tat , and rev . Use with envelope plasmids from Jakob Reiser. Jakob...Sergey Kasparov 22500 pNHP 2nd Contains gag , pol , rev , and tat . Sergey Kasparov Return to top Popular...of pLL3.7 with added genetic elements known to prevent epigenetic silencing. Expresses shRNA under mouse...

  2. p53 Pathway

    Type
    Collection
    ...suppression. Bieging KT, Mello SS, Attardi LD. Nat Rev Cancer. 2014 May;14(5):359-70. PubMed PMID: 24739573...from the mutant p53 field. Brosh R, Rotter V. Nat Rev Cancer. 2009 Oct;9(10):701-13. PubMed PMID: 19693097...p53 targets. Menendez D, Inga A, Resnick MA. Nat Rev Cancer. 2009 Oct;9(10):724-37. PubMed PMID: 19776742...

  3. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...AAV9 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-9-18917P 18917-AAV9 AAV-FLEX-rev-ChR2-tdtomato... James M. Wilson AV-1-18917P 18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Optogenetics Scott Sternson AV-1-20071P...Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-1-20949P 20949...GENIE, Douglas Kim AV-10-18917P 18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-10-PV1963 105542-... James M. Wilson AV-5-18917P 18917-AAV5 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-5-20071P 20071-AAV5...Pennsylvania Vector Core (Penn Vector Core) and Addgene previously partnered to provide high-quality AAV vectors...

  4. How to Write a Scientific Review Article

    Type
    Blog Post
    ...journal’s submission rules for review articles Whether submitting a review by invitation or by your own ...discussing the review topic with your peers. Start reading! I started by reading other reviews because, as...Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you ...you dread the thought of writing a review, or if you’re currently stuck trying to write one, hopefully this...field and are the perfect person to be writing the review! Doing so is a great way to develop your ability...information: basically, a scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with...thing I did was google “how to write a scientific review.” The second thing I did was stare in horror at...

  6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...co-founders of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering ... how feasible they were, conducting literature reviews and having weekly journal clubs outside of work...process, we had an idea that survived the literature review, survived technical feasibility analysis, and was...accomplish the color change until a few months later. RevBio accelerates with help from SynBio Axlr8r We limped... revbio...

  7. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ..., etc. Also, a constant in-flow of individuals wherever the cell cultures are kept will increase the risk...detection of mycoplasmas difficult (Ligasová, 2019). Prevention of mycoplasma contamination The best and the ...humans are the major source of contamination, the prevention steps start from you. If you are careful enough...contamination but it’s a very good practice for prevention. Prepare a small quantity of media stock that...that would be used for the experiment, preventing contamination of the rest. Avoid long exposure of the ...only be used to remove mycoplasma and not for preventing mycoplasma growth. Also, prolonged use of a particular...Conclusion To sum up, it is always important to prevent contamination rather than eradicating it later....

  9. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...pyogenes that locks itself together, to produce irreversible protein-peptide interactions. In a study published... peptide tags, which often form only weak and reversible bonds. Howarth’s team has already demonstrated...SpyCatcher system, to be able to grab on to proteins irreversibly in a genetically encoded way. SpyTag is nice...

  11. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...early peer-review attempts took place at the beginning of the 18th century. Yet the peer-review was not ...the DNA, were not peer-reviewed (Baldwin, 2015). At its very core, peer-review is a touchstone of the...peer-reviewer will help spot inconsistencies in the work, missing information that would prevent the study... easier for me to go back and finish the review.  Peer-review step 2: Detailed assessment After this initial...the peer-reviewer you wish for The last thing that I wanted to mention about the peer-review, is that ... in reviewing the paper of your colleagues. The young scientists that are reading your peer-review comments...perspective of an author submitting a paper, the peer-review seems like another dragon to slay on the way to...

  12. mTOR Pathway

    Type
    Collection
    ... L, Sonenberg N, Pelletier J, Topisirovic I. Nat Rev Drug Discov. 2015 Apr;14(4):261-78. doi: 10.1038/...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ... pRSV-Rev - This 3rd generation lentiviral packaging plasmid from the Trono lab contains the rev gene....Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation...pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral...contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2.G. Find ...contains all the necessary elements for packaging, reverse transcription, and integration needed for the production...

  14. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...plasmid for expressing a novel reverse transcriptase (RT), MarathonRT. Reverse transcriptases are critical...Plasmids Podcast!  MarathonRT: A highly processive reverse transcriptase Article contributed by Will Arnold...outperforms other RTs in this regard and is even able to reverse transcribe the extremely long, highly structured...techniques that are dependent on long, error-free, reverse transcription of RNA such as long-read cDNA sequencing...

  15. Chemogenetics AAV Preps

    Type
    Collection
    ...PHPeB Jordane Dimidschstein 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent...

  16. Viral Production

    Type
    Collection
    ...probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease... et al. (2014) (Link opens in a new window) ). Previous experience suggests that titers obtained using...

  17. Ras Pathway

    Type
    Collection
    ...signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...packaging plasmid encoding HIV proteins gag, pol, rev, and tat. 3rd generation systems require an envelope..., one encoding gag and pol and a second encoding rev.  Secondly, 3rd generation packaging systems have.... Third-generation vectors contain gag, pol, and rev. Second-generation contains those three genes plus...specific tissues are areas of active study (for a review click here). Alternative envelopes are powerful...the 3'LTR in the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target...

  19. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... HIV Tat gene (previously used to drive expression from the LTRs) has been removed, Rev (which facilitates...the production of a viral particle (Gag, Pol, Tat, Rev and Env) and the transfer plasmid encoding the insert... sequence, a part of the env gene containing the rev response element (RRE), an internal promoter, and...vector requires only three HIV-1 genes (gag, pol, and rev) for production. Third generation lentiviral vectors...cells transduced by a GFP encoded virus, and the reverse transcriptase activity assay uses qPCR to quantify... All of these methods have been described in a previous Addgene blog Once you know your viral titre, you...and U. Stein. "Viral vectors for gene transfer a review of their use in the treatment of human disease....

  20. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 61, 503-532. (1997). PubMed PMID:...10436161. 7. Dujon, B. Yeast evolutionary genomics. Nat Rev Genet 11, 512-524. (2010). PubMed PMID: 20559329....the S. cerevisiae lineage (7). In our class research project, students transform S. cerevisiae met deletion...synthesis (5). The budding yeast, Saccharomyces. cerevisiae, makes a great reference organism for the project... is available (6). Methionine synthesis in S. cerevisiae (Figure 1) occurs through a well-characterized...the same function as their counterparts in S. cerevisiae. The two yeasts are thought to have diverged ...carrying MET/Met genes from either S. pombe or S. cerevisiae. Students then determine if expression of the...
Showing: 1 - 20 of 741 results